Protein Info for Psyr_4320 in Pseudomonas syringae pv. syringae B728a

Annotation: ATP-dependent helicase HrpB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 844 TIGR01970: ATP-dependent helicase HrpB" amino acids 4 to 843 (840 residues), 1235.7 bits, see alignment E=0 PF00270: DEAD" amino acids 15 to 166 (152 residues), 40.6 bits, see alignment E=4.5e-14 PF04851: ResIII" amino acids 18 to 163 (146 residues), 22.8 bits, see alignment E=1.5e-08 PF00271: Helicase_C" amino acids 210 to 333 (124 residues), 50.3 bits, see alignment E=5.2e-17 PF08482: HrpB_C" amino acids 699 to 830 (132 residues), 181 bits, see alignment E=2.7e-57

Best Hits

KEGG orthology group: K03579, ATP-dependent helicase HrpB [EC: 3.6.4.13] (inferred from 100% identity to psb:Psyr_4320)

Predicted SEED Role

"ATP-dependent helicase HrpB"

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZNC2 at UniProt or InterPro

Protein Sequence (844 amino acids)

>Psyr_4320 ATP-dependent helicase HrpB (Pseudomonas syringae pv. syringae B728a)
MTSLPIDSVLPALRQALSTRHEAVLEAPPGAGKTTRVPLALLEETWLAGQTILMLEPRRL
AARAAAERLASELGEKVGETVGYRIRLESRVGPKTRIEVVTEGILTRRLQDDPALEGVGL
LIFDEFHERSLDADLALALSLNGRELFRDDQPLKILLMSATLEGERLAALLDDAPVVRSD
GRMFPVSMQWGRPFQPGEFVEPRVVQTVLDALGSESGSLLVFLPGQAEIRRVNQQLAEAL
GERADILLCPLHGELDLNAQRAAIEPAPKGTRKVVLATNIAETSLTIDGVRVVIDAGLAR
VPRFDPGSGMTRLETQRISRASATQRAGRAGRLEPGVCYRLWSEAQHDQLAAYGAAEILQ
ADLAGLALQLARWGVTPAQLVWLDVPPAAAYAQAQDLLVRLEALNNQPGQPPALTPHGQA
MAELPAHPRIAHLLLRGHALGLGELACDVAALLAERDILRGAGADLHSRLTLLAGTERAA
RGAQGGVQRARQLSRQYRGYLRGAANSPVSDPDHSRWLGALLALAYPDRVAQQRRAGGAE
YRLANGRAALFAEADALMKQPWLVIADLGSRQGQREERIYLAAEFDPALFDSVLAEQVIS
VDQIDWDEREGVFRAERQRKVGELIISREPLTGLDDATRSHALLALVRRKGLELLPWTAE
LRQWQARVALLRGLDIEKSSASEWPDLSDAQLLATLENWLMPYLGKVTRLSHFSQLDLSS
ILRNLLPWPLPQQLEAQAPQTLQVPSGSNIRIDYSEHPPILSVRLQELFGLSDTPRIANG
RQVLKLHLLSPARRPVQVTQDLANFWRSTYIEVKKDLKGRYPKHYWPDDPLVAEATARVK
PRGT