Protein Info for Psyr_4227 in Pseudomonas syringae pv. syringae B728a

Annotation: Dihydrodipicolinate synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 303 TIGR03249: 5-dehydro-4-deoxyglucarate dehydratase" amino acids 3 to 299 (297 residues), 435.6 bits, see alignment E=4.2e-135 PF00701: DHDPS" amino acids 12 to 298 (287 residues), 173.3 bits, see alignment E=2.5e-55

Best Hits

Swiss-Prot: 100% identical to KDGD_PSEU2: Probable 5-dehydro-4-deoxyglucarate dehydratase (Psyr_4227) from Pseudomonas syringae pv. syringae (strain B728a)

KEGG orthology group: K01707, 5-dehydro-4-deoxyglucarate dehydratase [EC: 4.2.1.41] (inferred from 99% identity to psp:PSPPH_4252)

MetaCyc: 66% identical to 5-dehydro-4-deoxyglucarate dehydratase subunit (Acinetobacter baylyi ADP1)
5-dehydro-4-deoxyglucarate dehydratase. [EC: 4.2.1.41]

Predicted SEED Role

"5-dehydro-4-deoxyglucarate dehydratase (EC 4.2.1.41)" in subsystem D-Galacturonate and D-Glucuronate Utilization or D-galactarate, D-glucarate and D-glycerate catabolism (EC 4.2.1.41)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.41

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZNL5 at UniProt or InterPro

Protein Sequence (303 amino acids)

>Psyr_4227 Dihydrodipicolinate synthetase (Pseudomonas syringae pv. syringae B728a)
MNPQELKSILSSGLLSFPVTDFNAQGDFHRAGYIKRLEWLAPYGASALFAAGGTGEFFSL
AASEYSEIIKTAVDTCATSVPILAGVGGATRQAIEYAQEAERLGAKGLLLLPHYLTEASQ
DGVAAHVEAVCKSVKIGVVVYNRNVCRLTPTLLEQLAERCPNLIGYKDGLGDIELMVSIR
RRLGDRFSYLGGLPTAEVYAAAYKALGVPVYSSAVFNFVPKLAMDFYHAIARDDHQAVGK
YIDDFFLPYLEIRNRKAGYAVSIVKAGAKIAGYDAGPVRAPLTDLTSDECDMLAALMDKQ
GKQ