Protein Info for Psyr_4180 in Pseudomonas syringae pv. syringae B728a

Annotation: bacterial translation initiation factor 2 (bIF-2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 841 PF04760: IF2_N" amino acids 1 to 51 (51 residues), 29.9 bits, see alignment 1.3e-10 amino acids 266 to 316 (51 residues), 59.4 bits, see alignment 8.4e-20 PF08364: IF2_assoc" amino acids 61 to 95 (35 residues), 41.3 bits, see alignment (E = 5e-14) TIGR00487: translation initiation factor IF-2" amino acids 260 to 840 (581 residues), 848.9 bits, see alignment E=2.4e-259 TIGR00231: small GTP-binding protein domain" amino acids 344 to 500 (157 residues), 100.4 bits, see alignment E=9.1e-33 PF00009: GTP_EFTU" amino acids 345 to 501 (157 residues), 126.4 bits, see alignment E=3.6e-40 PF01926: MMR_HSR1" amino acids 346 to 451 (106 residues), 36.4 bits, see alignment E=1.8e-12 PF11987: IF-2" amino acids 617 to 731 (115 residues), 133 bits, see alignment E=1.8e-42

Best Hits

Swiss-Prot: 100% identical to IF2_PSEU2: Translation initiation factor IF-2 (infB) from Pseudomonas syringae pv. syringae (strain B728a)

KEGG orthology group: K02519, translation initiation factor IF-2 (inferred from 100% identity to psb:Psyr_4180)

Predicted SEED Role

"Translation initiation factor 2" in subsystem NusA-TFII Cluster or Translation initiation factors eukaryotic and archaeal or Universal GTPases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZNR2 at UniProt or InterPro

Protein Sequence (841 amino acids)

>Psyr_4180 bacterial translation initiation factor 2 (bIF-2) (Pseudomonas syringae pv. syringae B728a)
MTQVTVKELAKVVDTPVERLLQQMREAGLSHTAAEQVVTDNEKQALLTHLKSGHKAKVEE
PRKITLQRKTTSTLRVAGSKSISVEVRKKKVFVQRSPEEIEAERKREMDERRAVENAARQ
KAEEEAKRRAEEDARSQPAASQSAPAAAEPVAAAEPVREAAPAAAPAPASAAPSADARKR
DEQRRPDKPRADDRNARGGDGERKNAPHRASVKEKAPAPRVAPRTTDEESDSFRRGGRGK
GKLKKRNAHGFQSPTGPVIRDVAIGETITVGELSAQMSVKAAEVIKFMFKMGTPVTINQV
LDQETAQLIAEELGHKVTLVSDNALEDSLAESLKFEGESFSRAPVVTVMGHVDHGKTSLL
DYIRRAKVAAGEAGGITQHIGAYHVETERGMVTFLDTPGHAAFTAMRARGAKATDIVILV
VAADDGVMPQTIEAVQHAVAAGVPLVVAVNKIDKPGADLDRIRSELSVHGVTSEEWGGDT
PFVSVSAKMGTGVDELLEAVLLQAEVLELKATPSAPGRGVVVESRLDKGRGPVATVLVQD
GTLRQGDMVLVGSNFGRIRAMLDENGKPVKEAGPSIPVEILGLDGTPDAGDEMSVLSDEK
KAREVALFRQGKFREVKLARAHAGKLENIFENMGQAEKKTLNIVLKSDVRGSLEALNGAL
NGLGNDEVQVRVVGGGVGGITESDANLALASNAVLFGFNVRADAGARKIVEQEGLDMRYY
NVIYDIIEDVKKALTGMLGSDVRENILGIAEVRDVFRSPKFGAIAGCMVLEGTVYRNRPI
RVLREDIVIFEGELESLRRFKDDAAEVRAGMECGIGVKSYNDVKVGDKIEVFEKVQVARS
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