Protein Info for Psyr_4010 in Pseudomonas syringae pv. syringae B728a

Annotation: Citrate-proton symport

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 433 transmembrane" amino acids 28 to 51 (24 residues), see Phobius details amino acids 60 to 82 (23 residues), see Phobius details amino acids 94 to 114 (21 residues), see Phobius details amino acids 120 to 141 (22 residues), see Phobius details amino acids 160 to 181 (22 residues), see Phobius details amino acids 193 to 212 (20 residues), see Phobius details amino acids 240 to 261 (22 residues), see Phobius details amino acids 281 to 300 (20 residues), see Phobius details amino acids 311 to 329 (19 residues), see Phobius details amino acids 335 to 358 (24 residues), see Phobius details amino acids 370 to 392 (23 residues), see Phobius details amino acids 404 to 422 (19 residues), see Phobius details PF00083: Sugar_tr" amino acids 23 to 219 (197 residues), 90.4 bits, see alignment E=1.9e-29 amino acids 223 to 419 (197 residues), 39 bits, see alignment E=7.2e-14 TIGR00883: MFS transporter, metabolite:H+ symporter (MHS) family protein" amino acids 28 to 417 (390 residues), 466 bits, see alignment E=5.5e-144 PF07690: MFS_1" amino acids 54 to 382 (329 residues), 95.2 bits, see alignment E=6.1e-31 amino acids 275 to 427 (153 residues), 42.4 bits, see alignment E=6.6e-15 PF12832: MFS_1_like" amino acids 261 to 416 (156 residues), 28.1 bits, see alignment E=1.6e-10

Best Hits

Swiss-Prot: 67% identical to KGTP_SHIFL: Alpha-ketoglutarate permease (kgtP) from Shigella flexneri

KEGG orthology group: K02625, MFS transporter, MHS family, dicarboxylic acid transporter PcaT (inferred from 100% identity to psb:Psyr_4010)

MetaCyc: 67% identical to alpha-ketoglutarate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-23

Predicted SEED Role

"dicarboxylic acid transporter PcaT" in subsystem Protocatechuate branch of beta-ketoadipate pathway

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZP82 at UniProt or InterPro

Protein Sequence (433 amino acids)

>Psyr_4010 Citrate-proton symport (Pseudomonas syringae pv. syringae B728a)
MTTTSEAPHYTGEERSKRIFAIVGASSGNLVEWFDFYIYAFCAIYFAPAFFPSDDPTVQL
LNTAGVFAAGFLMRPIGGWLFGRVADKHGRKNSMLISVTMMCAGSLIIACLPTYASIGAW
APALLLMARLLQGLSVGGEYGTTATYMSEVALRGQRGFYASFQYVTLIGGQLLAVLTVVI
LQQFLTTEELRDYGWRIPFVIGAGAAVIALLLRRTLNETTTAESRQDKDAGSIAALFKHH
AAAFITVLGYTAGGSLIFYTFTTYMQKYLVNTGGMEAKTASYIMTGALFLYMCMQPFFGM
LADRIGRRNSMLLFGALGTLCTVPILMTLKTTTNPFIAFVLITLALAIVSFYTSISGLVK
AEMFPPQVRALGVGLAYAVANAVFGGSAEWVALKLKSAGMENSFYWYVTAMMAVAFLFSL
RLPKEPKYLHQDH