Protein Info for Psyr_4008 in Pseudomonas syringae pv. syringae B728a

Annotation: Hydrophobe/amphiphile efflux-1 HAE1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1044 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details transmembrane" amino acids 339 to 359 (21 residues), see Phobius details amino acids 366 to 389 (24 residues), see Phobius details amino acids 395 to 416 (22 residues), see Phobius details amino acids 438 to 459 (22 residues), see Phobius details amino acids 471 to 495 (25 residues), see Phobius details amino acids 541 to 561 (21 residues), see Phobius details amino acids 872 to 890 (19 residues), see Phobius details amino acids 897 to 917 (21 residues), see Phobius details amino acids 923 to 947 (25 residues), see Phobius details amino acids 971 to 992 (22 residues), see Phobius details amino acids 1004 to 1029 (26 residues), see Phobius details TIGR00915: RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family" amino acids 1 to 1037 (1037 residues), 1806.7 bits, see alignment E=0 PF00873: ACR_tran" amino acids 1 to 1026 (1026 residues), 1348.2 bits, see alignment E=0

Best Hits

Swiss-Prot: 83% identical to ARPB_PSEPU: Antibiotic efflux pump membrane transporter ArpB (arpB) from Pseudomonas putida

KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 65% identity to abo:ABO_0964)

MetaCyc: 65% identical to multidrug efflux pump RND permease AcrB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-354; TRANS-RXN-355; TRANS-RXN-356; TRANS-RXN-357; TRANS-RXN-359; TRANS-RXN0-592

Predicted SEED Role

"RND efflux system, inner membrane transporter CmeB" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZP84 at UniProt or InterPro

Protein Sequence (1044 amino acids)

>Psyr_4008 Hydrophobe/amphiphile efflux-1 HAE1 (Pseudomonas syringae pv. syringae B728a)
MSKFFIDRPIFAWVIALVIMLVGALSISSLPINQYPSIAPPAIGIQVTYPGASAQTVQDT
VVQVIEQQLNGIDNLRYVSSESNSDGSMTITATFNQGTNPDTAQVQVQNKLNLATPLLPQ
EVQQQGIRVTKAVKNFLMVIGLVSEDGSMGKEDLANYIVSNMQDPISRTSGVGDFQVFGS
QYAMRIWLDPAKLNNFQLTPVDVKDAITAQNVQVSSGQLGGLPSISGQQLNATIIGKTRL
QTAEQFGNIFLKVNTDGSQVRLKDVATVGLGAENYSTDSQFDGKPASGLAIKLATGANAL
DTAKAIRATVSSLEPFFPPGMKVVYPYDTTPVVSESINGVVHTLIEAIVLVFLVMYLFLQ
NFRATIITTMTVPVVLLGTFGILAAFGFTINTLTMFGMVLAIGLLVDDAIVVVENVERVM
EEEKLSPRDATIKSMTQIQGALVGIALVLSAVLLPMAFFGGSTGVIYKQFSITIVSAMAL
SVVVALIFTPALCATMLKPIDHEKHGQPKRGFFGWFNRTFDRSVLSYERGVGNMLKHKWP
AYLGYILICAGMVFMFMRIPAAFLPEEDQGVIFAQIQTPAGSSTERTQEVIDQMREYLLT
KESGAVKSVFSVNGFNFAGRGQSSAIAFVMLKPWEERDSNNSVFELAKRAQGYFFSLRDA
MVFAVVPPSVLELGNATGFDVYLQDQGGVGHDKLMEARNQFLGMAAQSKILAGVRPNGLN
DEPQYQLIIDDERASALGITLSDINNTLSIALGGSYVNDFIDRGRVKKVYIQGDAGARMT
PEDLKKWYVRNSAGEMVPFSAFASGKWSYGSPKLSRYNGVAAEEVLGTPAPGYSSGDAMN
EVEALAKKLPQGIGISWTGLSYEERLSGSQAPALYALSLLVVFLCLAALYESWSIPIAVI
LVVPLGVIGALMATSLRGLSNDVFFQVGLLVTVGLAAKNAILIVEFAKELHEQGKSLVDA
AIEACRMRLRPIIMTSMAFILGVVPLAISSGAGSGSQHSIGTGVIGGMITAVILAIFWVP
LFFVTVSGLFKSKQKHISHDEAGQ