Protein Info for Psyr_3999 in Pseudomonas syringae pv. syringae B728a

Annotation: General substrate transporter:Major facilitator superfamily

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 441 transmembrane" amino acids 39 to 57 (19 residues), see Phobius details amino acids 69 to 90 (22 residues), see Phobius details amino acids 98 to 117 (20 residues), see Phobius details amino acids 123 to 144 (22 residues), see Phobius details amino acids 166 to 187 (22 residues), see Phobius details amino acids 199 to 218 (20 residues), see Phobius details amino acids 251 to 269 (19 residues), see Phobius details amino acids 288 to 308 (21 residues), see Phobius details amino acids 317 to 336 (20 residues), see Phobius details amino acids 342 to 366 (25 residues), see Phobius details amino acids 378 to 399 (22 residues), see Phobius details amino acids 411 to 429 (19 residues), see Phobius details PF00083: Sugar_tr" amino acids 32 to 239 (208 residues), 104.3 bits, see alignment E=7.4e-34 amino acids 224 to 435 (212 residues), 47.5 bits, see alignment E=1.3e-16 PF07690: MFS_1" amino acids 37 to 382 (346 residues), 104.6 bits, see alignment E=5.5e-34 amino acids 276 to 436 (161 residues), 41 bits, see alignment E=1.2e-14

Best Hits

KEGG orthology group: K03761, MFS transporter, MHS family, alpha-ketoglutarate permease (inferred from 100% identity to psb:Psyr_3999)

Predicted SEED Role

"Dicarboxylate MFS transporter"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZP93 at UniProt or InterPro

Protein Sequence (441 amino acids)

>Psyr_3999 General substrate transporter:Major facilitator superfamily (Pseudomonas syringae pv. syringae B728a)
METSNPASHGSAATAAGQKTTSSRLKSIFSGSVGNMVEWYDWYVYAAFSLYFAKAFFPKG
DTTAQLLNTAAIFAVGFLMRPIGGWLMGLYADYKGRKAALMASVLLMCFGSLIIALTPGY
ESIGVGAPILLVFARLLQGLSVGGEYGTSATYLSEMATKERRGFFSSFQYVTLISGQLIA
LGVLIVLQQTLTTEQLYAWGWRIPFVIGALCAIVALFLRRGMEETESFTRKKKEKPKESL
MRTLMRHPKELMTVVGLTMGGTLAFYTYTTYMQKYLVNTVGMSITDSTSISAATLFLFML
LQPVVGALSDKIGRRPILIAFGVLGTLCTVPILTTLHTIQTWWGAFFLIMAALIIVSGYT
SINAVVKAELFPTEIRALGVGLPYALTVSIFGGTAEYIALWFKSIGMETGYYWYVTACIA
CSLLVYVFMKDTRKHSRIETD