Protein Info for Psyr_3962 in Pseudomonas syringae pv. syringae B728a

Annotation: Glycine cleavage T protein (aminomethyl transferase)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 315 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details TIGR03317: folate-binding protein YgfZ" amino acids 172 to 237 (66 residues), 89.6 bits, see alignment E=4.7e-30

Best Hits

KEGG orthology group: K06980, (no description) (inferred from 100% identity to psb:Psyr_3962)

Predicted SEED Role

"Folate-dependent protein for Fe/S cluster synthesis/repair in oxidative stress"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZPD0 at UniProt or InterPro

Protein Sequence (315 amino acids)

>Psyr_3962 Glycine cleavage T protein (aminomethyl transferase) (Pseudomonas syringae pv. syringae B728a)
MTMAHTAFFCTLSHEGVLAVRGVDASKFLQGQLTCNLNYLNEDTSSLGARCTQKGRMQSS
FRIVFEGDGCLLAMAGELIEAQLLDLRKYAVFSKSKLTDESADWVRFGLQDGDAALVSLG
LDLPQQTDSVVRANDLMAIRVSPGRAELWVRSAEVDSIKSRLASHLNEAPLNDWLLGQIR
VGIGQVFGSTREEFIPQMINLQAVGGVSFKKGCYTGQEIVARMQYLGKLKRRLYRLTLSG
EEIPQPGTALFSPVHASAVGNVVIAAQDGQNIELLAVLQGDAAEDGRINLGSPEGAALQM
SELPYTLDSKLETQR