Protein Info for Psyr_3944 in Pseudomonas syringae pv. syringae B728a

Annotation: ATP dependent PIM1 peptidase, Serine peptidase, MEROPS family S16

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 805 PF02190: LON_substr_bdg" amino acids 40 to 236 (197 residues), 82.8 bits, see alignment E=9.5e-27 TIGR00763: endopeptidase La" amino acids 42 to 805 (764 residues), 903.7 bits, see alignment E=6.7e-276 PF07728: AAA_5" amino acids 387 to 520 (134 residues), 33.8 bits, see alignment E=9.4e-12 PF00004: AAA" amino acids 387 to 526 (140 residues), 90.5 bits, see alignment E=3.9e-29 PF22667: Lon_lid" amino acids 549 to 599 (51 residues), 64.1 bits, see alignment 2.2e-21 PF05362: Lon_C" amino acids 605 to 804 (200 residues), 258.6 bits, see alignment E=1.1e-80 PF13541: ChlI" amino acids 652 to 774 (123 residues), 34.1 bits, see alignment E=6.9e-12

Best Hits

Swiss-Prot: 62% identical to LON_MAGMM: Lon protease (lon) from Magnetococcus marinus (strain ATCC BAA-1437 / JCM 17883 / MC-1)

KEGG orthology group: K01338, ATP-dependent Lon protease [EC: 3.4.21.53] (inferred from 100% identity to psb:Psyr_3944)

Predicted SEED Role

"ATP-dependent protease La (EC 3.4.21.53) Type I" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent (EC 3.4.21.53)

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.53

Use Curated BLAST to search for 3.4.21.53

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZPE8 at UniProt or InterPro

Protein Sequence (805 amino acids)

>Psyr_3944 ATP dependent PIM1 peptidase, Serine peptidase, MEROPS family S16 (Pseudomonas syringae pv. syringae B728a)
MSDQQTDSPDLPNDADEHDVSLSSDSTSLALPGQNLPDKVYIIPIHNRPFFPAQVLPVIV
NEEPWAETLELVSKSEHHSLALFFMDTPQEDPRHFKTDALPEYGTLVKVHHASRENGRLQ
FVAQGLSRVRIRTWLKHHRPPYLVEVEYPQQPNEPTDEVKAYGMALINAIKELLPLNPLY
SEELKNYLNRFSPNDPSPLTDFAAALTSATGVELQEVLDCVPMLRRMEKVLPMLRKEVEV
ARLQKEISAEVNRKIGEHQRQFFLKEQLKVIQQELGLSKDDRSADIEQFEQRLEGKTLPP
QARKKFDEEIGKLKVLETGSPEYAVTRNYLDWTSSLPWGVYGADKLDLKHARKVLDQHHA
GLDDIKARILEFLAVGAYKGEISGSIVLLVGPPGVGKTSVGRSIAESLGRPFYRLSVGGM
RDEAEIKGHRRTYIGAQPGKLVQALKDVEVMNPVIMLDEIDKMGQSYQGDPASALLETLD
PEQNVEFLDHYLDLRLDLSKVLFVCTANTLDSIPGPLLDRMEVIRLSGYITEEKLAIAKR
HLWPKQLAKAGVAKNKLSISDSALRAVIEGYAREAGVRHLEKQLGKLVRKAVVKLLDEPD
SVIKIGPKDLEASLGMPVFRSEQVLSGTGVITGLAWTSMGGATLPIEATRIHTLNRGFKL
TGQLGDVMKESAEIAYSYVSANLSKFGGDAKFFDEAFVHVHVPEGATPKDGPSAGVTMAS
ALLSLARNQPPKKGVAMTGELTLTGHVLPIGGVREKVIAARRQKIHELILPEPNRGNFEE
LPDYLKEGITVHFAKRFADVVKVLF