Protein Info for Psyr_3698 in Pseudomonas syringae pv. syringae B728a

Annotation: Response regulator receiver:ATP-binding region, ATPase-like:Histidine kinase, HAMP region:Histidine kinase A, N-terminal:Hpt

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 917 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 168 to 188 (21 residues), see Phobius details PF09984: sCache_4" amino acids 35 to 168 (134 residues), 63.4 bits, see alignment E=6.2e-21 PF00672: HAMP" amino acids 190 to 240 (51 residues), 46.1 bits, see alignment 1.5e-15 PF00512: HisKA" amino acids 285 to 349 (65 residues), 82 bits, see alignment 7.6e-27 PF02518: HATPase_c" amino acids 396 to 511 (116 residues), 97.3 bits, see alignment E=2.3e-31 PF00072: Response_reg" amino acids 669 to 783 (115 residues), 97.5 bits, see alignment E=1.6e-31 PF01627: Hpt" amino acids 830 to 911 (82 residues), 51.2 bits, see alignment E=3.6e-17

Best Hits

Swiss-Prot: 100% identical to GACS_PSESY: Sensor protein GacS (gacS) from Pseudomonas syringae pv. syringae

KEGG orthology group: K07678, two-component system, NarL family, sensor histidine kinase BarA [EC: 2.7.13.3] (inferred from 80% identity to pfs:PFLU3777)

Predicted SEED Role

"BarA sensory histidine kinase (= VarS = GacS)" in subsystem Type III secretion system orphans

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZQ42 at UniProt or InterPro

Protein Sequence (917 amino acids)

>Psyr_3698 Response regulator receiver:ATP-binding region, ATPase-like:Histidine kinase, HAMP region:Histidine kinase A, N-terminal:Hpt (Pseudomonas syringae pv. syringae B728a)
MLTKLGIKGRVLLLTILPASLMAAMLGGYFTWMQLSELQSQLLQRGEMIAQDLAPLAANA
LGRKDKVLLSRIATQTLEQTDVRAVSFLDTDRTVLAHAGPTMISPSPIGSGSQLLSSTGT
DATRYLLPVFGSQRHLTSPIIPAEADTLLGWVELEISHNGTLLRGYRSLFASLLLILTGL
AFTATLAVRMSRTINGPMSQIKQAVSQLKDGNLETRLPPLGSRELDELASGINRMAATLQ
NAQEELQLSIDQATEDVRQNLETIEIQNIELDLARKEALEASRIKSEFLANMSHEIRTPL
NGILGFTHLLQKSELTPRQFDYLGTIEKSADNLLSIINEILDFSKIEAGKLVLDNIPFNL
RDLLQDTLTILAPAAHAKQLELVSLVYRDTPLALSGDPLRLRQILTNLVSNAIKFTREGT
IVARAMLEDETEEHAQLRISVQDTGIGLSSQDVRALFQAFSQADNSLSRQPGGTGLGLVI
SKRLIEQMGGEIGVDSTPGEGSEFWISLKLPKAREDKEESLNIPLGGLRAAVLEHHDLAR
QALEHQLEDCGLQTIVFNNLENLLNGVTAAHETPAAIDLAVLGVTALEISPERLRQHIWD
LENLNCKVMVLCPTTEHALFQLAVHDVYTQLQAKPACTRKLQKALSELIAPRAVRADIGP
PLSSRAPRVLCVDDNPANLLLVQTLLEDMGAEVVAVEGGYAAVNAVQQEAFDLVLMDVQM
PGMDGRQATEAIRAWEAERNQSSLPIVALTAHAMANEKRSLLQSGMDDYLTKPISERQLA
QVVLKWTGLALRNPAPERQNEALEVHVGPLVLDHEEGLRLAAGKADLAADMLAMLLASLD
ADREAIRVARANQDVHALIERIHRLHGATRYCGVPQLRSACQRAETLLKQNAPHTEEALN
DLDKAIIRLEAEARVMA