Protein Info for Psyr_3697 in Pseudomonas syringae pv. syringae B728a

Annotation: cysteine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01138: cysteine synthase B
  • amino acids 20 to 309 (290 residues), 530.6 bits, E=1.1e-163
• TIGR01136: cysteine synthase
  • amino acids 22 to 309 (288 residues), 401.7 bits, E=2.1e-124
• PF00291: PALP
  • amino acids 22 to 298 (277 residues), 246.9 bits, E=1.5e-77

Best Hits

Swiss-Prot: 86% identical to CYSM_PSEAE: Cysteine synthase B (cysM) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K12339, cysteine synthase B [EC: 2.5.1.47] (inferred from 100% identity to psb:Psyr_3697)

MetaCyc: 70% identical to cysteine synthase B (Escherichia coli K-12 substr. MG1655)
Cystathionine gamma-lyase. [EC: 4.4.1.1, 4.4.1.28]; Cysteine synthase. [EC: 4.4.1.1, 4.4.1.28, 2.5.1.47]; SULFOCYS-RXN [EC: 4.4.1.1, 4.4.1.28, 2.5.1.47, 2.5.1.144]

Predicted SEED Role

"Cysteine synthase (EC 2.5.1.47)" in subsystem Cysteine Biosynthesis or Methionine Biosynthesis (EC 2.5.1.47)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
• glycine betaine degradation III (7/7 steps found)
• D-serine degradation (3/3 steps found)
• L-serine degradation (3/3 steps found)
• L-methionine biosynthesis II (5/6 steps found)
• L-cysteine biosynthesis I (2/2 steps found)
• glycine betaine degradation I (6/8 steps found)
• L-cysteine biosynthesis VII (from S-sulfo-L-cysteine) (3/4 steps found)
• L-cysteine degradation II (2/3 steps found)
• L-methionine degradation II (2/3 steps found)
• L-tryptophan degradation II (via pyruvate) (2/3 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (7/10 steps found)
• L-threonine degradation I (4/6 steps found)
• seleno-amino acid biosynthesis (plants) (3/5 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• superpathway of L-cysteine biosynthesis (fungi) (3/6 steps found)
• felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis (2/5 steps found)
• superpathway of L-cysteine biosynthesis (mammalian) (2/5 steps found)
• L-mimosine degradation (4/8 steps found)
• homocysteine and cysteine interconversion (1/4 steps found)
• superpathway of L-threonine metabolism (11/18 steps found)
• glutathione-mediated detoxification I (3/8 steps found)
• L-cysteine biosynthesis VI (reverse transsulfuration) (2/7 steps found)
• hydrogen sulfide biosynthesis II (mammalian) (1/6 steps found)
• superpathway of L-methionine salvage and degradation (7/16 steps found)
• hypoglycin biosynthesis (4/14 steps found)
• superpathway of seleno-compound metabolism (6/19 steps found)

KEGG Metabolic Maps

• Cysteine metabolism
• Glycine, serine and threonine metabolism
• Methionine metabolism
• Nitrogen metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.47

Use Curated BLAST to search for 2.5.1.144 or 2.5.1.47 or 4.4.1.1 or 4.4.1.28

Sequence Analysis Tools

See Q4ZQ43 at UniProt or InterPro

Protein Sequence (313 amino acids)

>Psyr_3697 cysteine synthase (Pseudomonas syringae pv. syringae B728a)
MIGTPRSLDCEDAMTLQYQTIADCVGNTPLVRLQRMAGNTSNTLLLKLEGNNPAGSVKDR
PALSMITRAELRGQIHPGDTLIEATSGNTGIALAMAAAIKGYRMILIMPDNSSAERKAAM
TAYGAELISVSKDDGMEGARDLAERMQAEGRGKVLDQFANGDNPEAHYTSTGPEIWRQTG
GTVTHFVSSMGTTGTIMGTSRYLKEQNPDVQIVGLQPMEGASIPGIRRWPQEYLPKIYQS
DRVDRIIDMGQSEAEDTMRRLAREEGIFCGVSSGGSVAGALRISREVENAVIVAIICDRG
DRYLSTGVYDSPN