Protein Info for Psyr_3655 in Pseudomonas syringae pv. syringae B728a

Annotation: diguanylate cyclase/phosphodiesterase with PAS/PAC sensor(s)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 820 transmembrane" amino acids 12 to 36 (25 residues), see Phobius details amino acids 202 to 224 (23 residues), see Phobius details TIGR00229: PAS domain S-box protein" amino acids 250 to 366 (117 residues), 43.4 bits, see alignment E=3.6e-15 PF00989: PAS" amino acids 251 to 360 (110 residues), 41.8 bits, see alignment E=2.9e-14 PF08448: PAS_4" amino acids 251 to 364 (114 residues), 30 bits, see alignment E=1.6e-10 PF13426: PAS_9" amino acids 256 to 362 (107 residues), 35.4 bits, see alignment E=3.2e-12 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 370 to 534 (165 residues), 135.8 bits, see alignment E=1.2e-43 PF00990: GGDEF" amino acids 374 to 531 (158 residues), 140 bits, see alignment E=1.9e-44 PF00563: EAL" amino acids 553 to 793 (241 residues), 213.5 bits, see alignment E=8.9e-67

Best Hits

KEGG orthology group: None (inferred from 100% identity to psb:Psyr_3655)

Predicted SEED Role

"Sensory box/GGDEF family protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZQ85 at UniProt or InterPro

Protein Sequence (820 amino acids)

>Psyr_3655 diguanylate cyclase/phosphodiesterase with PAS/PAC sensor(s) (Pseudomonas syringae pv. syringae B728a)
MKQPWTFSKPRLLGIVWPFIAVALFQALLGCVSLYTMSAVRSYVAGESLWSKGQKDAIHY
LSLYANRHDERDYLKYQKAFSVPQGGHALRKALDQPIPSLADARGGIIQGGNHPDDVNGI
IWMYLNFHNFSFMKQAIHFWEVGDGYLMQLDELARHIHDRVGQGAVSAAEVDQWREQINV
INEGVTPAARAFSDALGEGSRLILNLLIAVNLITAVVLILLALLRVRRLIVQRRVFADAL
QLEKERAQITLESIGDGVITIDVAGAIVYMNPAAEGLTHWKGTQASGLPLAALFNLLDEN
DQKDQSTLVEQIITGKLKGGSANSKLIQRLDGSTVSVALVGTPIYTEGVISGAVLVLHDM
TQEQQYIADLSWQAAHDPLTGLVNRSEFERRLQRVLDEDCGQGRSHCLMFLDLDQFKLVN
DTGGHAAGDELLRQLCRALPQGLRDGDTLARLGGDEFGVLLVDCPAETALDMAEALREVV
QRLHFMWKGRPFMTTVSIGLVHLGDTPASLEGSLSAADMACYMAKERGRNRVQAYSVDDL
TLSMRFGEMAWIQRLHMAMDENRFCLYAQEIASIKGEVGEVRHIEILLRLRDESGRMVVP
DNFIPAAERYGLMTTLDRWVVHNVFKVIAQCQSDDRHQVPQALCAINLSGSSIGDDTFLE
YLKQQFKTFDVSPGMICFEITETSAIADLQSAIRFINELKALGCQFSLDDFCSGMSSFAY
LKHLPVDFLKIDGSFVKDMLDNPINKAMVEVINNIGHVMGKRTIAEYVETPQIESALQEM
GVDFAQGYLISRPELFTCESLYIQPVRPDPLMFGAPGTLR