Protein Info for Psyr_3647 in Pseudomonas syringae pv. syringae B728a

Annotation: DNA gyrase subunit A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 925 TIGR01063: DNA gyrase, A subunit" amino acids 8 to 639 (632 residues), 966.2 bits, see alignment E=6e-295 PF00521: DNA_topoisoIV" amino acids 32 to 508 (477 residues), 548.9 bits, see alignment E=9e-169 PF03989: DNA_gyraseA_C" amino acids 540 to 586 (47 residues), 34.6 bits, see alignment 1.1e-12 amino acids 591 to 639 (49 residues), 43.1 bits, see alignment 2.2e-15 amino acids 686 to 728 (43 residues), 31.2 bits, see alignment 1.2e-11 amino acids 732 to 777 (46 residues), 52.1 bits, see alignment 3.5e-18 amino acids 784 to 828 (45 residues), 43.1 bits, see alignment 2.3e-15 amino acids 833 to 879 (47 residues), 47.6 bits, see alignment 9.1e-17

Best Hits

KEGG orthology group: K02469, DNA gyrase subunit A [EC: 5.99.1.3] (inferred from 66% identity to abo:ABO_1751)

Predicted SEED Role

"DNA gyrase subunit A (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZQ93 at UniProt or InterPro

Protein Sequence (925 amino acids)

>Psyr_3647 DNA gyrase subunit A (Pseudomonas syringae pv. syringae B728a)
MGELAKEILPVNIEDELKQSYLDYAMSVIVGRALPDARDGLKPVHRRVLFAMSELGNDWN
KPYKKSARVVGDVIGKYHPHGDTAVYDTIVRMAQPFSLRYLLVDGQGNFGSVDGDNAAAM
RYTEVRMTKLAHELLADLHKETVDWVPNYDGTEMIPAVMPTRIPNLLVNGSSGIAVGMAT
NIPPHNLGEVIDGCLALIDNPELTVDELMQYIPGPDFPTAAIINGRAGIIEAYRTGRGRI
YMRARSIVEDIDKVGGRQQIVITELPYQLNKARLIEKIAELVKEKKLEGITELRDESDKD
GMRVVIELRRGEVPEVILNNLYAQTQLQSVFGINIVALIDGRPRILNLKDLLEAFVRHRR
EVVTRRTVFELRKARERGHILEGQAVALSNIDPVIALIKASPTPAEAKEALIKTPWESSA
VVEMVERAGADSCRPENLDPQYGLREGKYFLSPEQAQAILELRLHRLTGLEHEKLLGEYQ
EILNQIGELIRILNSATRLMEVIREELELIRSEYGDARRTEILDARLDLTLGDLITEEER
VVTISHGGYAKTQPLAVYQAQRRGGKGKSATGIKDEDYIAHLLVANSHTTLLMFSSKGKV
YWLKTYEIPEASRAARGRPLVNLLPLSDGEYITTMLPVDLEAMRKRADEEGEALEGELDD
AENSSETEEERKARIKAADKKKAPFIFMSTANGTVKKTPLVAFSRQRSSGLIALELDEGD
ILISAAITDGEQEIMLFSDGGKVTRFKESDVRAMGRTARGVRGMRLPEGQKLISMLIPEE
GSQILTASERGYGKRTAISEFPEYKRGGQGVIAMVSNERNGRLVGAVQVLDGEEIMLISD
QGTLVRTRVGEVSSLGRNTQGVTLIKLASDEKLVGLERVQEPSEVEGEELEGELVDGVIV
DAAEVDDAGEDLQADAASDEDEPQN