Protein Info for Psyr_3586 in Pseudomonas syringae pv. syringae B728a
Annotation: Phosphoesterase PHP, N-terminal:PHP, C-terminal
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 40% identical to RNAAM_ECOLI: 5'-3' exoribonuclease (yciV) from Escherichia coli (strain K12)
KEGG orthology group: K07053, (no description) (inferred from 100% identity to psb:Psyr_3586)MetaCyc: 40% identical to RNase AM (Escherichia coli K-12 substr. MG1655)
RXN-16009 [EC: 3.1.3.97]; 3.1.13.- [EC: 3.1.3.97]; RXN-24047 [EC: 3.1.3.97]
Predicted SEED Role
"COG0613, Predicted metal-dependent phosphoesterases (PHP family)"
Isozymes
No predicted isozymesUse Curated BLAST to search for 3.1.3.97
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q4ZQF4 at UniProt or InterPro
Protein Sequence (287 amino acids)
>Psyr_3586 Phosphoesterase PHP, N-terminal:PHP, C-terminal (Pseudomonas syringae pv. syringae B728a) MNVDLHCHSTASDGALSPTALVARAHENGVRVLSLTDHDTLEGLEEARAAAHSLGMQLVN GVELSCTWGGATIHILGYGFDTRAPALTEAISRLHEGRWLRAEEISRKLAIKGMPGALEG ARAIQQELGDSGNAPARPHFADFMVRSGFVKDRAEAFRKWLGAGKLGDVKQHWPTLEDTV ATLRASGAWVSLAHPSHYDFTRSKRRKLVGDFVQAGGHAIEVVNGMQPADQVGTLAILAR EFGLLVSAGSDFHGPGTWGEIGTYRPVPEDLPPLWCRFKHEQPTASV