Protein Info for Psyr_3574 in Pseudomonas syringae pv. syringae B728a

Annotation: Helix-turn-helix, Fis-type

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 525 PF00989: PAS" amino acids 84 to 156 (73 residues), 24.1 bits, see alignment E=7.7e-09 PF00158: Sigma54_activat" amino acids 208 to 374 (167 residues), 191.8 bits, see alignment E=1.9e-60 PF14532: Sigma54_activ_2" amino acids 211 to 379 (169 residues), 56.3 bits, see alignment E=1.1e-18 PF18024: HTH_50" amino acids 468 to 514 (47 residues), 54 bits, see alignment 2.7e-18 TIGR04381: TyrR family helix-turn-helix domain" amino acids 469 to 515 (47 residues), 85.1 bits, see alignment 1.2e-28

Best Hits

KEGG orthology group: K03721, transcriptional regulator of aroF, aroG, tyrA and aromatic amino acid transport (inferred from 100% identity to psb:Psyr_3574)

Predicted SEED Role

"Phenylalanine hydroxylase transcriptional activator PhhR" in subsystem Aromatic amino acid degradation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZQG6 at UniProt or InterPro

Protein Sequence (525 amino acids)

>Psyr_3574 Helix-turn-helix, Fis-type (Pseudomonas syringae pv. syringae B728a)
MRIKVHCQNRIGILRDILDLLVAYGVNVARGEVGGEHGDAIYLHCPNLINLQFQALRPKF
EAITGVFGVKRVGLMPSERRHMELNALLGALEFPVLSIDMGGAIVAANRAAAQLLGVRVD
EVPGMPLSRYAEDFDLPELVRASQSRINGLRVKVCGDVFLADIAPLQSEHDDSEAMAGAV
LTLHRADRVGERIYHVRKQELRGFDSIFQSSKVMAAVVREARRMAPLDAPLLIEGETGTG
KELLARACHLASPRGQSPLMALNCAGLSESMAEIELFGYDPGAFEGARAEGRLGLLELTA
GGTLFLDGVGELSARMQAKLLRFIQDGSFRRVGSDEDMFLDVRVMCSTQVDLSELCAKGM
FRQDLYHRLNVLSLHIPPLRDCLDGLEPLVEHFLDQASRQIGCALPGISPAAIKRLGQYH
WPGNVRQLENVLFQAVSLCDGGKVQAEHIRLPDYGMRQGLSEVSLEGGLGAIIGRFEKSV
LEQLYAQYPSSRQLGKRLGVSHTTIANKLRDYEVGRESDPHSTDK