Protein Info for Psyr_3556 in Pseudomonas syringae pv. syringae B728a

Annotation: alanyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01411: tRNA-synt_2c
  • amino acids 30 to 577 (548 residues), 744.8 bits, E=8.9e-228
• TIGR00344: alanine--tRNA ligase
  • amino acids 30 to 878 (849 residues), 1056.6 bits, E=0
• PF07973: tRNA_SAD
  • amino acids 676 to 720 (45 residues), 54.3 bits, 1.6e-18
• PF02272: DHHA1
  • amino acids 758 to 891 (134 residues), 95.8 bits, E=4.9e-31

Best Hits

Swiss-Prot: 66% identical to SYA_MARHV: Alanine--tRNA ligase (alaS) from Marinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)

KEGG orthology group: K01872, alanyl-tRNA synthetase [EC: 6.1.1.7] (inferred from 82% identity to avn:Avin_34460)

MetaCyc: 65% identical to alanine--tRNA ligase/DNA-binding transcriptional repressor (Escherichia coli K-12 substr. MG1655)
Alanine--tRNA ligase. [EC: 6.1.1.7]; 6.1.1.7 [EC: 6.1.1.7]; 6.1.1.7 [EC: 6.1.1.7]; RXN-19726 [EC: 6.1.1.7, 3.1.1.96]; 3.1.1.- [EC: 6.1.1.7, 3.1.1.96]

Predicted SEED Role

"Alanyl-tRNA synthetase (EC 6.1.1.7)" (EC 6.1.1.7)

MetaCyc Pathways

• tRNA charging (20/21 steps found)
• superpathway of chorismate metabolism (42/59 steps found)
• shinorine biosynthesis (3/8 steps found)
• vanchrobactin biosynthesis (2/8 steps found)
• enterobactin biosynthesis (4/11 steps found)
• ergotamine biosynthesis (4/12 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
• bacillibactin biosynthesis (3/12 steps found)
• superpathway of ergotamine biosynthesis (5/18 steps found)
• sulfazecin biosynthesis (1/16 steps found)
• phosalacine biosynthesis (4/25 steps found)
• phosphinothricin tripeptide biosynthesis (4/25 steps found)
• corallopyronin A biosynthesis (2/30 steps found)
• colibactin biosynthesis (6/38 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Aminoacyl-tRNA biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.1.96 or 6.1.1.7

Sequence Analysis Tools

See Q4ZQI4 at UniProt or InterPro

Protein Sequence (899 amino acids)

>Psyr_3556 alanyl-tRNA synthetase (Pseudomonas syringae pv. syringae B728a)
MRPFAVATSFDVLHLPLAAVSVEEPMKSAEIREAFLGFFEEQGHTRVASSSLIPGNDPTL
LFTNAGMNQFKDCFLGQEKRAYTRAVTSQKCVRAGGKHNDLENVGYTARHHTFFEMLGNF
SFGDYFKRDAITYAWTFLTSEKWLNLPKEKLWVTVYATDDEAYDIWTKEIGVPAERMVRI
GDNKGAPYASDNFWTMGDTGPCGPCSEIFFDHGPEIWGGPPGSPEEDGDRYIEIWNNVFM
QFNRTADGVLHPLPAPSVDTGMGLERVSAVLQHVHSNYEIDLFQSLLAASAKAIGCSNDN
QASLKVVADHIRSCGFLIADGVLPSNEGRGYVLRRIIRRACRHGNKLGAKGSFFYQIVAA
LVAEMGSAFPELVQQQSHIERVLKGEEEQFAKTLEQGLKILEQDLADLKGTVVPGEVVFK
LYDTYGFPMDLTGDIARERNLTLDEAGFEREMDAQRVRARSASSFGMDYNSLVKVDVATQ
FTGYSATTGSASVVALYKEGQSVSHLNEGEEGVVILDITPFYAESGGQIGDSGFLQAGDA
RFDVSDTTKTGGAFLHHGVVASGSLSVGAQVETQVADEVRDATKLNHSATHLLHAALRQV
LGEHVQQKGSLVDSQRLRFDFSHFEAIKAEQLRALEDIVNAEIRKNTEVMTEETDIDTAK
KKGAMALFGEKYGDSVRVLSMGGEFSVELCGGIHASRTGDIALFKIVSEGGVAAGVRRIE
AVTGAAALAWLNSAEDQLKEAATLVKGNRDNLLDKLTAVIERNRLLEKQLEQLQAKAASA
AGDDLSSAALDVKGVKVLATRLDGQDGKALLALVDQLKNKLGRAVILLGSVHEDKVVLVA
GVTKDLTGQLKAGDLMKQAAAAVGGKGGGRPDMAQGGGVDAGALDSALALAVPFVEQGI