Protein Info for Psyr_3525 in Pseudomonas syringae pv. syringae B728a

Annotation: 3-hydroxyisobutyrate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 296 PF02826: 2-Hacid_dh_C" amino acids 4 to 72 (69 residues), 25.6 bits, see alignment E=2.2e-09 PF02737: 3HCDH_N" amino acids 4 to 44 (41 residues), 25.1 bits, see alignment 4.5e-09 PF03446: NAD_binding_2" amino acids 5 to 162 (158 residues), 136.1 bits, see alignment E=3.6e-43 PF03807: F420_oxidored" amino acids 5 to 96 (92 residues), 29.6 bits, see alignment E=2.7e-10 PF14833: NAD_binding_11" amino acids 166 to 285 (120 residues), 128.8 bits, see alignment E=4e-41

Best Hits

Swiss-Prot: 34% identical to HMGD_EUBBA: 2-(hydroxymethyl)glutarate dehydrogenase (Hgd) from Eubacterium barkeri

KEGG orthology group: K00020, 3-hydroxyisobutyrate dehydrogenase [EC: 1.1.1.31] (inferred from 100% identity to psb:Psyr_3525)

MetaCyc: 34% identical to 2-(hydroxymethyl)glutarate dehydrogenase subunit (Eubacterium barkeri)
2-hydroxymethylglutarate dehydrogenase. [EC: 1.1.1.291]

Predicted SEED Role

No annotation

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.31

Use Curated BLAST to search for 1.1.1.291 or 1.1.1.31

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZQL5 at UniProt or InterPro

Protein Sequence (296 amino acids)

>Psyr_3525 3-hydroxyisobutyrate dehydrogenase (Pseudomonas syringae pv. syringae B728a)
MTLKAGVIGLGNMGGGMAATLAGKGFDVSGFDLSQAALAQAESKGVKPVADRTQLIQGVD
ILILSLPKAEHVESVCLGAGGINEVGRKGLIVVDTTTSTPEMSRKVAAELAKNGIAFIDA
PVSGGPKGAATGSMSMVIGAEDADLARAMPVLEGMSGTRVHVGQCGAGNVAKIANNMLAA
CHLISTAEAVAMAARAGVDPEKLLQGLNAGSGRSGATQVMFPTWVLNKAYDSGFTMGLMR
KDVGLASDLADSLDMDLPLSRVVAQLWQASSETLADNEDFCAIVQRTDGKLYGHGE