Protein Info for Psyr_3498 in Pseudomonas syringae pv. syringae B728a

Annotation: Conserved hypothetical protein 730

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 468 PF14793: DUF4478" amino acids 21 to 127 (107 residues), 141 bits, see alignment E=2.7e-45 PF03641: Lysine_decarbox" amino acids 203 to 342 (140 residues), 94.9 bits, see alignment E=9.8e-31 PF11892: PpnN_C" amino acids 347 to 467 (121 residues), 190.4 bits, see alignment E=2e-60

Best Hits

Swiss-Prot: 57% identical to PPNN_ECOLI: Pyrimidine/purine nucleotide 5'-monophosphate nucleosidase (ppnN) from Escherichia coli (strain K12)

KEGG orthology group: K06966, (no description) (inferred from 100% identity to psb:Psyr_3498)

MetaCyc: 57% identical to nucleotide 5'-monophosphate nucleosidase (Escherichia coli K-12 substr. MG1655)
AMP nucleosidase. [EC: 3.2.2.4]; Inosinate nucleosidase. [EC: 3.2.2.4, 3.2.2.12]; Pyrimidine-5'-nucleotide nucleosidase. [EC: 3.2.2.4, 3.2.2.12, 3.2.2.10]; 3.2.2.- [EC: 3.2.2.4, 3.2.2.12, 3.2.2.10]

Predicted SEED Role

"Decarboxylase family protein"

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.2.4

Use Curated BLAST to search for 3.2.2.10 or 3.2.2.12 or 3.2.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZQP2 at UniProt or InterPro

Protein Sequence (468 amino acids)

>Psyr_3498 Conserved hypothetical protein 730 (Pseudomonas syringae pv. syringae B728a)
MLDCIALMENRMIPRQVINATVSPKGSLETLSQREVQQLSAAGSGSTYTLFRQCALAILN
TGAHVDNAKTILEAYESFEVRIHQQDRGVRLELLNAPADAFVDGEMIASTREMLFSALRD
IVYTESELDSQRIDLSNSQGITDYVFHLLRNARTLRAGVEPKMVVCWGGHSINTEEYKYT
KKVGHELGLRSLDICTGCGPGVMKGPMKGATIAHAKQRIVGGRYLGLTEPGIIAAEAPNP
IVNELVILPDIEKRLEAFVRVGHGIIIFPGGAGTAEEFLYLLGILMHPDNKDVPFPVILT
GPKNTEPYLQQLHAFVGATLGEEAQRHYQIIIDNPADVARQMTQGLKEVKQFRRERNDAF
HFNWLLKIEESFQHPFDPTHENMSKLQLNHDVPTHELAANLRRAFSGIVAGNVKDKGIRL
IEQHGPYQIHGDPSIMGPLDKLLQAFVDQHRMKLPGGAAYVPCYQVVT