Protein Info for Psyr_3472 in Pseudomonas syringae pv. syringae B728a

Annotation: Mannosyl-glycoprotein endo-beta-N-acetylglucosamidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 414 PF10135: Rod-binding" amino acids 54 to 105 (52 residues), 55.2 bits, see alignment 8e-19 TIGR02541: flagellar rod assembly protein/muramidase FlgJ" amino acids 243 to 399 (157 residues), 203.4 bits, see alignment E=3.7e-64 PF01832: Glucosaminidase" amino acids 261 to 399 (139 residues), 123.3 bits, see alignment E=9.4e-40

Best Hits

KEGG orthology group: K02395, flagellar protein FlgJ (inferred from 100% identity to psb:Psyr_3472)

Predicted SEED Role

"Flagellar protein FlgJ [peptidoglycan hydrolase] (EC 3.2.1.-)" in subsystem Flagellum (EC 3.2.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.-

Use Curated BLAST to search for 3.2.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZQR8 at UniProt or InterPro

Protein Sequence (414 amino acids)

>Psyr_3472 Mannosyl-glycoprotein endo-beta-N-acetylglucosamidase (Pseudomonas syringae pv. syringae B728a)
MDMPKSGISSAVDSGAYTDVNRLASLKHGDKDSVENQKKVAREFESLFVSQMLKAMRSAN
EVLAKDNPMNTPATRQYQDMYDQQLAVTLSTRGNGIGLQDVLMRQLSKDKGINHAAPVNT
TDAATAATDAAPAKTGLATSVYQRPLWATRSVAADQAAAAASASGEGRNDMAMLNARRLS
LPAKLTDRLLAGIVPSATTAVSNSPVPARATAGTTGNNGDWTLDPNLAPAPEYVRSMAQP
PLAPAKRAFSNADQFVETMLPLAKEAAARIGVDPVMLVAQAALETGWGKSIMRQQDGSSS
HNLFGIKAAGSWKGAEARAITSEFRDGKMVKETADFRSYDSYADSFHDLVSLLQNNSRYK
EVVNSADKPEQFVKELQKAGYATDPDYASKISQIAKQMKSYQTYAAAAGSSTTL