Protein Info for Psyr_3461 in Pseudomonas syringae pv. syringae B728a

Annotation: Helix-turn-helix, Fis-type

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 491 PF06490: FleQ" amino acids 6 to 123 (118 residues), 98.7 bits, see alignment E=6e-32 PF00158: Sigma54_activat" amino acids 146 to 313 (168 residues), 257.2 bits, see alignment E=1.5e-80 PF14532: Sigma54_activ_2" amino acids 147 to 317 (171 residues), 77.2 bits, see alignment E=3.8e-25 PF07728: AAA_5" amino acids 170 to 288 (119 residues), 30.8 bits, see alignment E=6.7e-11 PF02954: HTH_8" amino acids 441 to 478 (38 residues), 42.3 bits, see alignment 1.3e-14

Best Hits

KEGG orthology group: K10941, sigma-54 specific transcriptional regulator, flagellar regulatory protein A (inferred from 100% identity to psb:Psyr_3461)

Predicted SEED Role

"Flagellar regulatory protein FleQ" in subsystem Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZQS9 at UniProt or InterPro

Protein Sequence (491 amino acids)

>Psyr_3461 Helix-turn-helix, Fis-type (Pseudomonas syringae pv. syringae B728a)
MWREIKILLIDDDSQRRRDLAVILNFLGEENLSCSSQDWQQVVGSLASPREVLCVLVGSV
NAPGSLQGLLKTIAAWDEFLPVLLMSENSSVELPEDLRRRVLSALEMPPSYSKLLDSLHR
AQVYREMYDQARERGRHREPNLFRSLVGTSRAIQHVRQMMQQVADTDASVLILGESGTGK
EVVARNLHYHSKRRDAPFVPVNCGAIPAELLESELFGHEKGAFTGAITSRAGRFELANGG
TLFLDEIGDMPLPMQVKLLRVLQERTFERVGSNKTQSIDVRIIAATHKNLETMIELGSFR
EDLYYRLNVFPIEMAPLRERVEDIPLLMNELISRMEHEKRGSIRFNSAAIMSLCRHAWPG
NVRELANLVERMAIMHPYGVIGVAELPKKFRYVDDEDEQMVDSMRSEIEERVAINSNTPN
FASGAMLPPEGLDLKDYLGGLEQGLIQQALDDANGIVARAAERLRIRRTTLVEKMRKYGM
SRREGDEQADD