Protein Info for Psyr_3454 in Pseudomonas syringae pv. syringae B728a

Annotation: ATPase FliI/YscN

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 452 TIGR01026: ATPase, FliI/YscN family" amino acids 27 to 446 (420 residues), 575.6 bits, see alignment E=6.5e-177 TIGR03496: flagellar protein export ATPase FliI" amino acids 29 to 444 (416 residues), 644.6 bits, see alignment E=6e-198 PF00006: ATP-synt_ab" amino acids 155 to 366 (212 residues), 296.3 bits, see alignment E=1.4e-92 PF18269: T3SS_ATPase_C" amino acids 373 to 443 (71 residues), 71.6 bits, see alignment E=4e-24

Best Hits

Swiss-Prot: 86% identical to FLII_PSEAE: Flagellum-specific ATP synthase (fliI) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02412, flagellum-specific ATP synthase [EC: 3.6.3.14] (inferred from 100% identity to psb:Psyr_3454)

Predicted SEED Role

"Flagellum-specific ATP synthase FliI" in subsystem Flagellar motility or Flagellum

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.14

Use Curated BLAST to search for 3.6.3.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZQT6 at UniProt or InterPro

Protein Sequence (452 amino acids)

>Psyr_3454 ATPase FliI/YscN (Pseudomonas syringae pv. syringae B728a)
MRLDRTSFAKRLGSYAESISLPAQPVVEGRLLRMVGLTLEAEGLRAAMGSRCVVINDDSH
HPVEVEAEVMGFSGGKVFLMPVGSVAGIAPGARVVPLADTGRLPMGMSMLGRVLDGAGRP
LDGRSPIKAEDWVPMDGPTINPLKRDPISQPLDVGIRCINGLLTVGRGQRLGLFAGTGVG
KSVLLGMMTRFTEADIIVVGLIGERGREVKEFIEHILGEEGLKRSVVVASPADDAPLMRL
RAAMYCTRIAEYFRDKGKNVLLLMDSLTRFAQAQREIALAIGEPPATKGYPPSVFARLPK
LVERAGNAEKGGGSITAFYTVLSEGDDQQDPIADSARGVLDGHIVLSRRLAEEGHYPAID
IEASISRVMPAVVSPEHMARAQHFKQLWSRYQQTRDLISVGAYVAGGDRETDMAIALHPV
LVRYQRQALRENESMQGSSEALASIFAPAPGG