Protein Info for Psyr_3380 in Pseudomonas syringae pv. syringae B728a

Annotation: Secretion protein HlyD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 294 transmembrane" amino acids 12 to 34 (23 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 46 to 214 (169 residues), 110.4 bits, see alignment E=4.4e-36 PF16576: HlyD_D23" amino acids 46 to 237 (192 residues), 41.4 bits, see alignment E=1.5e-14 PF13533: Biotin_lipoyl_2" amino acids 47 to 95 (49 residues), 65.2 bits, see alignment 5.3e-22 PF13437: HlyD_3" amino acids 155 to 237 (83 residues), 46.2 bits, see alignment E=9.7e-16

Best Hits

Swiss-Prot: 41% identical to AAEA_PECAS: p-hydroxybenzoic acid efflux pump subunit AaeA (aaeA) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: None (inferred from 100% identity to psb:Psyr_3380)

MetaCyc: 40% identical to aromatic carboxylic acid efflux pump membrane fusion protein (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-233

Predicted SEED Role

"Membrane fusion component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZR10 at UniProt or InterPro

Protein Sequence (294 amino acids)

>Psyr_3380 Secretion protein HlyD (Pseudomonas syringae pv. syringae B728a)
MKKPILTLGRVVLTLLVVTLAAVVVWRMVMYYMYAPWTRDGHIRADVVQIAPDVSGLIQK
VEVTDNQPVHKGQILFTIDQDRFRLALRQAQATVAERQETWEQARRENKRNRGLGNLVAR
EQLEESQSREARALSALGESKVAVDAAQLNLDRSVIRSPVDGYLNDRAPREHEFVTAGHP
VLSVVDSTSFHIDGYFEETKLDGIHVGQGVDIRVIGDSARLTGHVVSIVAGIEDRDRSSG
SNLLPNVNPAFSWVRLAQRIPVRIAFDDVPADFRMIAGRTATVSIIDDTQERSR