Protein Info for Psyr_3337 in Pseudomonas syringae pv. syringae B728a

Annotation: gluconate kinase, SKI family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 175 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details TIGR01313: carbohydrate kinase, thermoresistant glucokinase family" amino acids 11 to 170 (160 residues), 190.7 bits, see alignment E=7.9e-61 PF13671: AAA_33" amino acids 12 to 144 (133 residues), 28.2 bits, see alignment E=1e-10

Best Hits

Swiss-Prot: 47% identical to GCNK_GLUOX: Gluconokinase (GOX1709) from Gluconobacter oxydans (strain 621H)

KEGG orthology group: K00851, gluconokinase [EC: 2.7.1.12] (inferred from 100% identity to psb:Psyr_3337)

MetaCyc: 40% identical to D-gluconate kinase, thermosensitive (Escherichia coli K-12 substr. MG1655)
Gluconokinase. [EC: 2.7.1.12]

Predicted SEED Role

"Gluconokinase (EC 2.7.1.12)" in subsystem D-gluconate and ketogluconates metabolism or Entner-Doudoroff Pathway (EC 2.7.1.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZR53 at UniProt or InterPro

Protein Sequence (175 amino acids)

>Psyr_3337 gluconate kinase, SKI family (Pseudomonas syringae pv. syringae B728a)
MTTANTAISALVVMGVSGCGKSAVGAEIALNSGGRLIEGDAFHPPANIEKMSAGTPLNDE
DRAGWLTRLGEEMAAALAKGEHPVLTCSSLKLIYRQRLRDAVPGLGFVFLELSKELAAER
CSHRPGHFMPASLVDSQFATLEPPYGEPLTLIVDASQPIDVIGKQAAAWWKGSHA