Protein Info for Psyr_3269 in Pseudomonas syringae pv. syringae B728a

Annotation: 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 645 TIGR04377: 3,5/4-trihydroxycyclohexa-1,2-dione hydrolase" amino acids 5 to 643 (639 residues), 963.9 bits, see alignment E=2e-294 PF02776: TPP_enzyme_N" amino acids 7 to 134 (128 residues), 60.7 bits, see alignment E=1.9e-20 PF00205: TPP_enzyme_M" amino acids 220 to 353 (134 residues), 117.4 bits, see alignment E=5.8e-38 PF02775: TPP_enzyme_C" amino acids 441 to 600 (160 residues), 120.7 bits, see alignment E=7.4e-39

Best Hits

Swiss-Prot: 67% identical to IOLD_GEOKA: 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase (iolD) from Geobacillus kaustophilus (strain HTA426)

KEGG orthology group: K03336, 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase [EC: 3.7.1.-] (inferred from 100% identity to psb:Psyr_3269)

MetaCyc: 57% identical to 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase (Bacillus subtilis subtilis 168)
RXN-14149 [EC: 3.7.1.22]

Predicted SEED Role

"Epi-inositol hydrolase (EC 3.7.1.-)" in subsystem Inositol catabolism (EC 3.7.1.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.7.1.- or 3.7.1.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZRC1 at UniProt or InterPro

Protein Sequence (645 amino acids)

>Psyr_3269 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase (Pseudomonas syringae pv. syringae B728a)
MSTTRLTMAQALVKFLDNQYVEVDGVQSKFVAGIFTIFGHGNVLGLGQALEQDSGDLVVH
QGRNEQGMCHAAIGFAKQHLRRKIYACSSSVGPGAANMVTAAATASANRIPLLLLPGDVY
ASRQPDPVLQQIEQFHDLSISTNDAFKAVSKYWDRINRPEQLMSAALNAMRVLTDPADTG
AVTLALPQDVQAEAYDYPDSFLQKRVHRIDRRPPSKAMLDDALQLLVGKRKPLLICGGGV
RYSGAADALQAFAERFDIPFAETQAGKSAIVSAHPLNMGGIGETGTLAANRLAKEADLII
GVGTRYSDFTTASKWLFQNPDVQFLNLNVGAFDVQKLDGVQVLADAQTALQALTESLQAR
GYRAAWGDAPQSARAELHAEVDRLYAVEYQNEDFVPEINDHLDPAVLREFIELTGSCLTQ
SGVLGILNQSLPADAVIVAAAGSLPGDLQRAWRSTGVDTYHVEYGYSCMGYEVNAALGVK
LAAPHREVFALVGDGSYMMLHSELATSIQERRKINVVLLDNMTFGCINNLQMEHGMNSFG
TEFRFRNPDTGRLDGDFVPVDFAMSAAAYGCKTYKVNTAEQLRNALADAQRQTVSTLIDI
KVLPKTMIHKYLSWWRVGVAEVSTTGTTAQVYEKLNRELAKARQY