Protein Info for Psyr_3226 in Pseudomonas syringae pv. syringae B728a

Annotation: Transcriptional activator RfaH

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 170 PF02357: NusG" amino acids 9 to 102 (94 residues), 93.4 bits, see alignment E=5.2e-31 TIGR01955: transcription elongation factor/antiterminator RfaH" amino acids 10 to 163 (154 residues), 211.2 bits, see alignment E=4.1e-67

Best Hits

KEGG orthology group: K05785, transcriptional antiterminator RfaH (inferred from 100% identity to psb:Psyr_3226)

Predicted SEED Role

"Transcriptional activator RfaH" in subsystem Transcription factors bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZRG4 at UniProt or InterPro

Protein Sequence (170 amino acids)

>Psyr_3226 Transcriptional activator RfaH (Pseudomonas syringae pv. syringae B728a)
MSHSDQDVRWYLIQTKPRQEARAEEHLRRQQFECYRPLQADAKKTGTRVQAGEPLFPGYL
FIHMDQVQDNWYPIRSTRGVSRIVTFGGHPVPVRDHLIEQIRQRLATPAPKTQFAQGDAV
LITHASWGDVEAIFLSKDGTQRAVILLNMLQRQQKVVLPISSLSRIEARA