Protein Info for Psyr_3167 in Pseudomonas syringae pv. syringae B728a

Annotation: Citrate-proton symport

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 433 transmembrane" amino acids 31 to 50 (20 residues), see Phobius details amino acids 56 to 81 (26 residues), see Phobius details amino acids 91 to 114 (24 residues), see Phobius details amino acids 123 to 145 (23 residues), see Phobius details amino acids 166 to 184 (19 residues), see Phobius details amino acids 190 to 209 (20 residues), see Phobius details amino acids 238 to 262 (25 residues), see Phobius details amino acids 278 to 296 (19 residues), see Phobius details amino acids 308 to 330 (23 residues), see Phobius details amino acids 339 to 359 (21 residues), see Phobius details amino acids 371 to 393 (23 residues), see Phobius details amino acids 404 to 422 (19 residues), see Phobius details PF00083: Sugar_tr" amino acids 22 to 219 (198 residues), 82.3 bits, see alignment E=3.7e-27 amino acids 225 to 422 (198 residues), 40.1 bits, see alignment E=2.2e-14 PF07690: MFS_1" amino acids 24 to 375 (352 residues), 90.9 bits, see alignment E=8.2e-30

Best Hits

Swiss-Prot: 77% identical to CITH_KLEPN: Citrate-proton symporter (citH) from Klebsiella pneumoniae

KEGG orthology group: K03288, MFS transporter, MHS family, citrate/tricarballylate:H+ symporter (inferred from 100% identity to psb:Psyr_3167)

MetaCyc: 69% identical to propane-1,2,3-tricarboxylate-proton symporter (Salmonella enterica enterica serovar Typhimurium str. LT2)
RXN1R65-49

Predicted SEED Role

"metabolite/H symporter, major facilitator superfamily (MFS)"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZRM3 at UniProt or InterPro

Protein Sequence (433 amino acids)

>Psyr_3167 Citrate-proton symport (Pseudomonas syringae pv. syringae B728a)
MHGSSSLRKESSRSKAGAVFRVTSGNFLEQFDFFLFGFYATQIASAFFPASSEFASLMMT
FAVFGAGFLMRPLGAVILGAYIDDVGRRKGLIVTLSIMASGTLLIVLVPGYASIGLWAPA
LVLVGRLLQGFSAGAELGGVSVYLAEMATPGRKGFYTSWQSGSQQVAIVVAAALGYGLNQ
WMAPAAVADWGWRIPFAIGCLIIPFIFLLRRSLQETEEFATRSHRPTMRQVFATLLQNWR
VVIAGMLMVAMTTTTFYLITIYAPTFGKTVLNLSTADALLVTLLVGVSNFIWLPVGGMLS
DRFGRKPLLVAMTLLTIISAYPALSFLALAPSFGHMLEVLLWFSFLYGLYNGAMIPALTE
IMPVEVRVAGFSLAYSLATAVFGGFTPAISTWLIHLTGDKAAPAYWMTFAAACALGATLV
LYRHISTRPQLAV