Protein Info for Psyr_3153 in Pseudomonas syringae pv. syringae B728a

Annotation: Beta-glucosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 913 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF00933: Glyco_hydro_3" amino acids 95 to 313 (219 residues), 85.7 bits, see alignment E=8.3e-28 PF01915: Glyco_hydro_3_C" amino acids 348 to 755 (408 residues), 187.5 bits, see alignment E=5.6e-59 PF07691: PA14" amino acids 435 to 591 (157 residues), 76.2 bits, see alignment E=5e-25 PF14310: Fn3-like" amino acids 792 to 862 (71 residues), 89.9 bits, see alignment 1.8e-29

Best Hits

KEGG orthology group: K01188, beta-glucosidase [EC: 3.2.1.21] (inferred from 100% identity to psb:Psyr_3153)

Predicted SEED Role

"Beta-glucosidase (EC 3.2.1.21)" in subsystem Beta-Glucoside Metabolism or Fructooligosaccharides(FOS) and Raffinose Utilization (EC 3.2.1.21)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.21

Use Curated BLAST to search for 3.2.1.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZRN7 at UniProt or InterPro

Protein Sequence (913 amino acids)

>Psyr_3153 Beta-glucosidase (Pseudomonas syringae pv. syringae B728a)
MNKRKMMGAHSALALLALAVSQVHAADPTVQQGREDRAEKAAQKTLAKMTMEEKLAYIGG
TGGWDVKPLTNYGVPQIHGADGGVGVRYTSEGNDQGVVYPSGPNLAATFNPRRAIDLGRA
LGYDTAVGGYQFITGPGVNLYRMPYGGRAFEYLSGEDPFLGASLAPGVINGIQSRGVWAN
AKHYAANDQESNRFNLNQTMPERVLREMSLPAFESSSKNGNVAMMMCAFQKVNGDFACES
DHLIAQILKKEWGFKGFVQSDYNAVVHGFNAARAGTDLDMMGYQMNSSVLKPHLDAGDLS
AATIDDKVRRILKQIYLYKFDSKTPLTTHNMNSSTSNKVALNAAREGIVLLKNQDNLLPL
DKQKVKKIAVVGTLAKYSPPTGFGSANVMASHYVSELSGLQQIAPNAKVDFIEGLSLDPS
TSAWTTTDATGNEVQGMKVEYFSNTNWSGDAAVTRTEKHVDLDWANDKNLPFESNTSTSD
PYASKGSTAGQLNGDTSSTSIRYTGKVTPTQSGEQVFKVRADGAVRLWVNGKKIIDNGDG
KPLPGNSIPPTIPEFAKINLEAGQSYDVKLEYSRRAGYLSTMGGLVGVQLSSASLNAPQD
LSGYDAVVVAVGNSNEYEGEGFDHSFDLPEFQNELIQNIAKVNPNTVVTMYGGTGLKMSD
WIEQVPAALHAFYPGQNGGQALAEILFGKVNPSGKLPISIERNIEDNPAYASFPKFDNQN
TLTDMDYKDDLLLGYRGYEKKGIKPLYPFGYGLSYTTFGYSNIKVTPGVAVGNTPIKVSF
DLSNTGKVGGSEVAQLYVGQQNPKVERPIKELKGYKKVFLKPGESKRVTIELNDRSLAYF
DEKSKQWVVDADTFNISLGSSSQDIRLNAKLVNSFRQELSTTSSNPLPRSALNSVLVEKP
PVKTGGVFKQTVE