Protein Info for Psyr_3151 in Pseudomonas syringae pv. syringae B728a

Annotation: type II secretion system protein E (GspE)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 585 PF05157: MshEN" amino acids 64 to 170 (107 residues), 53.8 bits, see alignment E=2e-18 TIGR02533: type II secretion system protein E" amino acids 103 to 580 (478 residues), 647.5 bits, see alignment E=6.6e-199 PF00437: T2SSE" amino acids 207 to 473 (267 residues), 355.4 bits, see alignment E=1.7e-110

Best Hits

Swiss-Prot: 64% identical to GSPE_XANCP: Type II secretion system protein E (xpsE) from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)

KEGG orthology group: K02454, general secretion pathway protein E (inferred from 100% identity to psb:Psyr_3151)

Predicted SEED Role

"General secretion pathway protein E"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZRN9 at UniProt or InterPro

Protein Sequence (585 amino acids)

>Psyr_3151 type II secretion system protein E (GspE) (Pseudomonas syringae pv. syringae B728a)
MSLRSSKMPSFMPSAPVDVSRLLIPDAQQVCAWLIENAGLKTADLERAQRLQQESEGTEL
LGLLTRLGLVSEFELARAWAALLQAPLLLADAVPPLLDPLPPLTERFMRHYQVVPVGWSD
QGLQVLSANPGTLYPFQAIAYACEVPVRLSIGPRNEVETLIERYYGQGRSAMGTLIENLD
EEGGSLEDIEHLKDLASEAPVIRLVNLILQRAVEQRASDIHIEPFESQLKVRYRIDGVLH
EAEAPPSSSSAAVISRVKIMARLDIAERRLPQDGRIMLRIQGKELDLRVSTVPTSFGESV
VMRLLDRQTINFDFPSLGFDGERLDEFLDVLERPHGILLVTGPTGSGKTTTLYTALSRLN
TAERKIITVEDPVEYQLEGINQIQVKPSIGLDFAGALRSIVRQDPDVIMIGEMRDLETCR
IAIQSSLTGHLVLSTLHTNSAAASITRLLDMGVESYLIASTVNGILAQRLVRRLDPATRE
AFDAPPELIAEHGLERFTDERPIRLYRPRADAPGGGYRGRSAITELLVMNEELRSLLMRH
ADASTLEEAARRGGLRTLHEEGLRQAVAGVTSLEEVLRVTRGEGT