Protein Info for Psyr_3106 in Pseudomonas syringae pv. syringae B728a

Annotation: phosphate ABC transporter substrate-binding protein, PhoT family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 339 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF12849: PBP_like_2" amino acids 25 to 305 (281 residues), 130 bits, see alignment E=7.4e-42 TIGR00975: phosphate ABC transporter, phosphate-binding protein PstS" amino acids 27 to 336 (310 residues), 460.8 bits, see alignment E=1e-142

Best Hits

Swiss-Prot: 64% identical to PSTS_XYLFA: Phosphate-binding protein PstS (pstS) from Xylella fastidiosa (strain 9a5c)

KEGG orthology group: K02040, phosphate transport system substrate-binding protein (inferred from 100% identity to psb:Psyr_3106)

MetaCyc: 51% identical to phosphate ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-27-RXN [EC: 7.3.2.1]; 7.3.2.1 [EC: 7.3.2.1]

Predicted SEED Role

"Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism (TC 3.A.1.7.1)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.3.2.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZRT4 at UniProt or InterPro

Protein Sequence (339 amino acids)

>Psyr_3106 phosphate ABC transporter substrate-binding protein, PhoT family (Pseudomonas syringae pv. syringae B728a)
MILLTKKRLSLLIASLCLSGVAHATDVTGAGSSFVYPVISAWSQDYSKSKDNRINYQSIG
SGGGIAQIKAATVDFGASDAPMSAEDLQAGGLGQFPSVIGGIVPIVNVEGVESGELKLDG
PTIAKIFMGEIKKWNDPAITALNSGVKLPDTAITVVHRSDGSGTTYNFTNYLSKVSPEWN
EKLKFGSTVQWPAGVGGKGNEGVSAYVKQIKGSIGYVEYSYATTNKLSYTQLKNAAGKFI
KPDAKAFAAAADTADWASAKDFNLIMTNAPGEAAWPITATTWIIMYKKAKNEEKSTAAFD
FFKWSLENGQKQAEKLEYVALPKSLVDRVESYWKTEFTK