Protein Info for Psyr_3103 in Pseudomonas syringae pv. syringae B728a

Annotation: phosphate ABC transporter ATP-binding protein, PhoT family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 259 TIGR00972: phosphate ABC transporter, ATP-binding protein" amino acids 13 to 259 (247 residues), 384.1 bits, see alignment E=1.3e-119 PF00005: ABC_tran" amino acids 28 to 184 (157 residues), 120.5 bits, see alignment E=1.7e-38 PF13304: AAA_21" amino acids 107 to 213 (107 residues), 28.9 bits, see alignment E=2.2e-10

Best Hits

Swiss-Prot: 100% identical to PSTB1_PSEU2: Phosphate import ATP-binding protein PstB 1 (pstB1) from Pseudomonas syringae pv. syringae (strain B728a)

KEGG orthology group: K02036, phosphate transport system ATP-binding protein [EC: 3.6.3.27] (inferred from 100% identity to psp:PSPPH_3015)

MetaCyc: 67% identical to phosphate ABC transporter ATP binding subunit (Escherichia coli K-12 substr. MG1655)
ABC-27-RXN [EC: 7.3.2.1]; 7.3.2.1 [EC: 7.3.2.1]

Predicted SEED Role

"Phosphate transport ATP-binding protein PstB (TC 3.A.1.7.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism (TC 3.A.1.7.1)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.27

Use Curated BLAST to search for 3.6.3.27 or 7.3.2.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZRT7 at UniProt or InterPro

Protein Sequence (259 amino acids)

>Psyr_3103 phosphate ABC transporter ATP-binding protein, PhoT family (Pseudomonas syringae pv. syringae B728a)
MNNLSLANEKTKIQVRGLEFFYNNQKSLKSIDMTIPEKRITAIIGPSGCGKSTLLRVFNR
IYAMYPKQEARGEVLLNGENILAPGYSMNRLRSHVGMVFQKPVPFPMSIYDNISYAIKHH
EKLSRREMEDRVEQALRGAALWDEVKDKLKQSATGLSGGQQQRLCIARTIALRPQVLLLD
EPTSALDPISTGRIEQLITELKEQFTVIIVTHNMQQAARCSDYTAFMFMGELIEHGDTDT
IFTKPSKTQTEDYITGRFG