Protein Info for Psyr_3099 in Pseudomonas syringae pv. syringae B728a

Annotation: assimilatory nitrite reductase (NAD(P)H) large subunit precursor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 853 PF07992: Pyr_redox_2" amino acids 13 to 293 (281 residues), 171.2 bits, see alignment E=1.1e-53 TIGR02374: nitrite reductase [NAD(P)H], large subunit" amino acids 14 to 807 (794 residues), 1042.8 bits, see alignment E=0 PF00070: Pyr_redox" amino acids 155 to 223 (69 residues), 49.4 bits, see alignment E=1.6e-16 PF18267: Rubredoxin_C" amino acids 327 to 395 (69 residues), 33.1 bits, see alignment E=1.3e-11 PF04324: Fer2_BFD" amino acids 428 to 476 (49 residues), 35.5 bits, see alignment 3e-12 PF03460: NIR_SIR_ferr" amino acids 566 to 628 (63 residues), 45.1 bits, see alignment 2e-15 PF01077: NIR_SIR" amino acids 638 to 759 (122 residues), 77 bits, see alignment E=3.5e-25

Best Hits

Swiss-Prot: 61% identical to NIRB_KLEOX: Nitrite reductase [NAD(P)H] large subunit (nasB) from Klebsiella oxytoca

KEGG orthology group: K00362, nitrite reductase (NAD(P)H) large subunit [EC: 1.7.1.4] (inferred from 100% identity to psb:Psyr_3099)

Predicted SEED Role

"Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.1.4

Use Curated BLAST to search for 1.7.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZRU1 at UniProt or InterPro

Protein Sequence (853 amino acids)

>Psyr_3099 assimilatory nitrite reductase (NAD(P)H) large subunit precursor (Pseudomonas syringae pv. syringae B728a)
MNTTVFPLNDVKTLIVVGNGMVGHHCVEQLIAGGALERYRIHVFGEETQRAYDRVHLSEY
FTGRDAESLAMSEMSVYEQPGLTLHLGVPVLEIDRDRHEIITAEGCFSYDKLILATGSYP
FVPPIEGARGNSRLVYRTLEDLDSIRYAATKARRGVVVGGGLLGLEAANALKSLGLEAHV
VEFAPRLMPVQLDDLGGAALKARIEALGVGVHLSRATQSISDGKQYRYRMNFANDEFLET
DLIVFSAGIRPQDALARQCELALGPRGGIAIDEHCRTSDADIYAIGECAAWNGSVFGLVA
PGYQMARGVAAQLCEQDTDAFFGADMSTKLKLLGVDVGSIGDAHGALAGAVSYRFIDEAT
ASYRRLVVSADGKQVLGAVLVGDNSYYDTLLQYAQNGIPLPADPSSLILPRGEGAPTLGA
DALPATATICSCHNVSKGAVCSAIDNGCTDLAAIKSCTKAATGCGGCTALLKQVFEHELI
ARGVAVDKSLCEHFAYTRQELYSMVRVEGIETFDELLTRHGKGTHGCDICKPAVGSILAS
CWNRPITEPSLVPLQDTNDTFMANMQKNGTYSVVPRIPGGEITPDGLIAIGAVAKKYDLY
TKITGGQRIDLFGAQLHELPDIWSELIEAGFETGHAYGKSTRTVKSCVGSTWCRYGVQDS
VAMALRIEDRYKGLRSPHKLKFAVSGCTRECAEAQSKDVGVIATENGWNLYLCGNGGMRP
RHAELFATDLDDQTLIRYIDRFLMLYIRTADKLQRTSVWRETLEGGLDYLKAVIVDDSLG
LAAELESQMQLVVDRYECEWANALKDPEKLKRFRTFVNDGRADPDVQFVKERAQRRPARP
EELALIPLFKEVV