Protein Info for Psyr_3089 in Pseudomonas syringae pv. syringae B728a

Annotation: Hemolysin-type calcium-binding region:hemolysin-type calcium binding related protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 200 400 600 800 1000 1200 1400 1600 1800 2000 2200 2400 2689 PF00353: HemolysinCabind" amino acids 493 to 528 (36 residues), 29.4 bits, see alignment (E = 6.5e-11) amino acids 503 to 537 (35 residues), 35.1 bits, see alignment (E = 1e-12) amino acids 520 to 551 (32 residues), 23.2 bits, see alignment (E = 5.4e-09) amino acids 539 to 572 (34 residues), 30.2 bits, see alignment (E = 3.5e-11) amino acids 711 to 738 (28 residues), 8.6 bits, see alignment (E = 0.0002) amino acids 736 to 768 (33 residues), 29.1 bits, see alignment (E = 7.9e-11) amino acids 751 to 779 (29 residues), 18.2 bits, see alignment (E = 2e-07) amino acids 797 to 831 (35 residues), 24.9 bits, see alignment (E = 1.6e-09) amino acids 806 to 840 (35 residues), 24.7 bits, see alignment (E = 1.9e-09) amino acids 881 to 913 (33 residues), 34.4 bits, see alignment (E = 1.7e-12) amino acids 906 to 934 (29 residues), 24 bits, see alignment (E = 3e-09) amino acids 943 to 967 (25 residues), 18.1 bits, see alignment (E = 2.2e-07) amino acids 950 to 980 (31 residues), 22.2 bits, see alignment (E = 1.2e-08) amino acids 994 to 1023 (30 residues), 25.7 bits, see alignment (E = 9.2e-10) amino acids 1032 to 1064 (33 residues), 37.7 bits, see alignment (E = 1.6e-13) amino acids 1077 to 1108 (32 residues), 34.6 bits, see alignment (E = 1.5e-12) amino acids 1120 to 1152 (33 residues), 26.6 bits, see alignment (E = 4.8e-10) amino acids 1153 to 1185 (33 residues), 20.7 bits, see alignment (E = 3.3e-08) amino acids 1280 to 1314 (35 residues), 25.4 bits, see alignment (E = 1.2e-09) amino acids 1432 to 1465 (34 residues), 24.7 bits, see alignment (E = 1.8e-09) amino acids 1457 to 1492 (36 residues), 37.9 bits, see alignment (E = 1.4e-13) amino acids 1467 to 1501 (35 residues), 40.9 bits, see alignment (E = 1.5e-14) amino acids 1484 to 1515 (32 residues), 27.9 bits, see alignment (E = 1.9e-10) amino acids 1833 to 1866 (34 residues), 27.7 bits, see alignment (E = 2.1e-10) amino acids 2080 to 2107 (28 residues), 9.8 bits, see alignment (E = 8.7e-05) amino acids 2212 to 2242 (31 residues), 14.5 bits, see alignment (E = 3e-06) amino acids 2349 to 2377 (29 residues), 8.4 bits, see alignment (E = 0.00024) amino acids 2370 to 2403 (34 residues), 29.6 bits, see alignment (E = 5.3e-11) amino acids 2379 to 2410 (32 residues), 21.4 bits, see alignment (E = 2e-08) amino acids 2526 to 2559 (34 residues), 32.3 bits, see alignment (E = 7.5e-12) PF06594: HCBP_related" amino acids 2445 to 2480 (36 residues), 39.2 bits, see alignment (E = 5.4e-14)

Best Hits

Predicted SEED Role

"Alkaline phosphatase (EC 3.1.3.1)" in subsystem Phosphate metabolism (EC 3.1.3.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.1

Use Curated BLAST to search for 3.1.3.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZRV1 at UniProt or InterPro

Protein Sequence (2689 amino acids)

>Psyr_3089 Hemolysin-type calcium-binding region:hemolysin-type calcium binding related protein (Pseudomonas syringae pv. syringae B728a)
MNIQAYAQNSLLAQASYGLFDLLTNSDVIRALIAPDNGGFSETQATDFVESYKLVSYQQN
TENGFAAAIFENRITHEVTFSIRGTEGDFNDMVEADAIGIGFNGIATWQSIDLFNYYQEL
TTPAGQAYTAYSYSSGIYTPLGLTIVPAGVTTEVKIAGDGYKLSSSGFNIAGHSLGGHLS
IVLSRLAEDSVKSVFTYNAPGFDTALVPAANNTEWLFNTLAALQVAETGATSIGTKFSPE
KITNLVSPGDMVSKIGTVPGTIYKSFDELSDIGGAHSIVNLSDALAVASLLHFFDSSLTL
GAIAGILSSSTLDENLALDSVVSALNAIINSSPVKEPARVSELSRDDMYKNILSMENHWV
AGDKPTTFVTSLVGLSAGAIESLMYSNVAYKYAAYNFNGFALKNDSFYQGMQGIDTLNDY
NQATGTGNITSAWVSDRALYLSLLLKAGAEGADTALGSPLITLSKHTFLFEEGNTTYNTL
YVNEGIDRQSILFGSQADDNLLGSLARDHLYGESGNDYLYGGDGDDLLEGGEGNDHLNGG
RDDDRLIGMGGADTLTGGSGNDQLEGGAGIDTYSFFKGDGLDHIIDGDGLIKINEAAVHG
GKQVGPGANTWLSETGDVRFSLSDEGGDRKSLSMFYGDGDRIVIDNFVMGTFGITLSDYI
EPDRSLGNLNTIEGDLKPEDQDVTEADIQVGLDDWGNEIVMPGVADPDRENRLFDTPGND
NLLGLGGDDELTSRQGGDDRLDGGSGDDTLTGGVGDDTLVGGVGADILISSDGDDELYAE
VETTLDDFIMSTSPKNGANFQDWLDAGKGDDLLIGSSSRDMLLGGEGADTLAGGNGDDFL
GGDEIGGIPNADWSFTTDLQTNGLMLLKTFKAGLNDDIEDGGDDVMYGGAGNDMLWGSFG
NDYLSGDSGNDILDGNDGNDTLFGGTGDDFLAGDSRDASGNLGKGADYLDGGDGNDQLIG
SALNDVLIGGDGNDTLWGDNFFGADNLPGQDFLDGGDGSDYLMGGQDNDTLYGGTGEDFL
FGDDPSVPKAQQGNDLLYGGEGNDEIQGMGGNDSLYGGSENDTLLGDGRDVAAESHGDDL
IFGEAGNDSLWGGGGSDSLYGGEGADFLEGDFPGLTTLYEGNDYLDGGNGEDTLQGAGGD
DTLFGGNDNDVLFGGMGDNVLEGGQGNDFISASTGNDVYLFNSGDGADTIEDSGGNNKIF
FGGKFSVDSLEVRISTPSLGQQYLHVGDSLGSYILLANMPAWSTSSFSFVDGATLSYAEL
IKLSENSLKYTGFEKSELVYGTMLADVINGEGGDDSLNGQGGDDLLDGGEGADTYIVNAN
DGDDIISDSVWVPASTVTPPTIEQTTNAVAADSDISGNVVRFGQGITRDNVLFKEALTFS
LQKVLLVEYPGGSLTILGGFESVIAKYSFADGSVLTHADVMKIVSSTPAVSSNGADTITG
TDADDQLDGKDGADLIYGGLGNDSIVGGQGDDTLYGGQGDDHLYGGDGDDRLDGGSGNDV
LEGGAGDNTFYFSRGMENDTFIADGASNNTLIFGSDYKLSDVLITQSGADLVLTSRHGPE
SVTLKDYYTLHQSWSAVLAGVTYKGFDQILEASAFQETSAYASYQQQFIDSVQNDLFLRL
TSDVYTSLGDGNFQWVSRGDNAFSSWVSYNNWNSEFVIGHVEAPVGSMHHKQPAVAVLFE
DTSSSKRVENSAVISVAPSHSTGSNFVPKSGAVFHPSVSNTGFDFALGSTIVEVNDVNGK
PDGWWVYPPANSASSNSYKSIPEGSTQISKLFSVSTSLTTFTHKIVYGDNVSGRVDVEHG
NIFYAGSGDDLLVAYALYAYHDDLWYGGTMMSGGDGNDTLVGHHGDDVLIGGGGNNVFFG
GNGKDTYVIDGSAGTDIISDTPEPDDHDSWTADNLHIWTFGYETYSLDAADTVILPDGVT
IADLVLTQSVVVSEGVSLDLLKTPDAQYSGAVEAAKAAMAYNTLDLSWGGHKVQILLPHA
NDPRGSGIELIRFADGTEISPNDLMAQKGLFGVPDVYLAGANVDYRGLTSNRIETSLNFM
GGLGNDTVTTNKYDVYGFDGDDYLETVANKDWFGVGPGLYGGKGNDTLVVNSVGSTVEGG
EGDDVIVLSGAGMVNITGAVEGHDNILFRNGITAERLSFHKFESSLAILVDSDFNKVVLV
ENYFAASTLGDYTVRTADGAIYNKEDVGAMIAELPVPNAMLGGSAGNDIFFGSDAPEDLY
GSTGIDEVYGGGGSDRYFFSSGEYLAVHEVSGSNDTVVFDRDIDINLIASSVKKINNDLL
FDFNAVSAGRVTVKNFFLGGEFLVEKFMTQAGDVVPAQKIFDMFNIAMPLMSSPAYDSRI
NDLPDTDSVINGSDVRDDIFGFNGSDTLLGRGGDDRLSGGNGNDLLSGGLGNDRLIGGRG
DDVYVFAKGDGQDVIDNAGGGKDILRLVGINGADISGGLVKDANNLIINVHGSSDSITLE
NWFGGDGNVIENIIFDDGAVSSAAIASAYGIDIFSGEVPDVYAELPEERDYSNIVLAKQS
NTYMRGTQSSDFIEGASGNDVLDGSGGDDYLIGGRGNDMYLNIFAGDTGKDIINNYSGTP
SDSDSLMLGVADVRTVWFAKNNADLIISHLGTDNSVTIADWYLGTDYKLDQVTNGRYSLS
AEGVEDMVEVMSTLGQPVNGLITLAGDQQAVLDSLIASNWINYSTPVVM