Protein Info for Psyr_3055 in Pseudomonas syringae pv. syringae B728a
Annotation: Aminoglycoside phosphotransferase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: None (inferred from 100% identity to psb:Psyr_3055)Predicted SEED Role
"Choline kinase (EC 2.7.1.32)" in subsystem Phosphorylcholine incorporation in LPS (EC 2.7.1.32)
MetaCyc Pathways
- prenylated FMNH2 biosynthesis (3/3 steps found)
- cell-surface glycoconjugate-linked phosphocholine biosynthesis (1/3 steps found)
- phosphatidylcholine biosynthesis I (1/3 steps found)
- superpathway of phosphatidylcholine biosynthesis (1/12 steps found)
- plasmalogen biosynthesis I (aerobic) (2/16 steps found)
- superpathway of phospholipid biosynthesis II (plants) (10/28 steps found)
- type IV lipoteichoic acid biosynthesis (S. pneumoniae) (1/18 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.7.1.32
Use Curated BLAST to search for 2.7.1.32
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q4ZRY3 at UniProt or InterPro
Protein Sequence (317 amino acids)
>Psyr_3055 Aminoglycoside phosphotransferase (Pseudomonas syringae pv. syringae B728a) MSYADDLTTRLRSALTSCGFAADAGIENVTQGVASPSRLSTEWAGFRVSSAQGACYVKVL HADMAPLIDFDNVAQASECAGRSAAAPRLLGADQANGVLIFEDLGVDWRAARLDDLLAPG RLQALWALKRQVHAGPVPDVVRSPMADIERLRAVCERDNVALPAEHQWIDRCVDMAWQSL QSRSVQAVPLHGDGVSSNVMVSSEGQLRLVDFDYGGCMDPWYDVAITLNELYSFERQWRE GIGAWAGQCLEIDYAVCRLYALINDWYWTLWGLWAGTTSSRPLEFSKVGQWTLLRCRQCV QDPRLEGWMRQVQEGRA