Protein Info for Psyr_3035 in Pseudomonas syringae pv. syringae B728a

Annotation: Glycoside hydrolase, family 3, N-terminal:Glycoside hydrolase, family 3, C-terminal:Glycoside hydrolase, family 3, C-terminal

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 913 signal peptide" amino acids 1 to 42 (42 residues), see Phobius details PF00933: Glyco_hydro_3" amino acids 85 to 323 (239 residues), 116.4 bits, see alignment E=3.8e-37 PF01915: Glyco_hydro_3_C" amino acids 360 to 765 (406 residues), 181.4 bits, see alignment E=4.2e-57 PF07691: PA14" amino acids 452 to 610 (159 residues), 111.8 bits, see alignment E=5.1e-36 PF14310: Fn3-like" amino acids 802 to 872 (71 residues), 88.1 bits, see alignment 7e-29

Best Hits

KEGG orthology group: None (inferred from 100% identity to psb:Psyr_3035)

Predicted SEED Role

"Beta-glucosidase (EC 3.2.1.21)" in subsystem Beta-Glucoside Metabolism or Fructooligosaccharides(FOS) and Raffinose Utilization (EC 3.2.1.21)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.21

Use Curated BLAST to search for 3.2.1.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZS03 at UniProt or InterPro

Protein Sequence (913 amino acids)

>Psyr_3035 Glycoside hydrolase, family 3, N-terminal:Glycoside hydrolase, family 3, C-terminal:Glycoside hydrolase, family 3, C-terminal (Pseudomonas syringae pv. syringae B728a)
MNNLNQPITSTARTLALSAFKQTLGLSLLTFSVMQANLALAAVTPATGNEVEARVSSILD
NMNQSEKINFTRVNDGHMIPSLLKWGIKGTVAYDSSMGVHVNNATFGAQYPSQSALAATW
SINRAKEFGLAIAYETRISGGQQMLSPGVNLYRTPYNGRSAEYVSGEDPFLGAVLAPAIV
NGIQAQGIQASGKHYLANEQEANRQAIDVKVDERTLRELYLPGFESMVKNANVASIMCGF
NKVNGDYACENHHLITEVLKGEWGYQGMVISDFNAIHDAFKGAWAGTDIDMPSGLQFTEA
NLLPYLWSGQLTQNVIDDKVKRNLRGIVSYDLQNHLNTAKTLEHPEYGMRAALNTARESI
VLLRNENTAAGKPLLPLERSAKIAVIGNWAHDVPASPFGTANSPPNSYVTELSGLQQLAS
SSSDVTYLSEMSLNPASSVWYQPATGDNGISNAGVKAEYFSNTSFSGDPVLTRVEPGVNL
NWTTGSNVTNAGSTAVSGFSPTAGAFSARFTTTIKPTISGAQAFKVRADGPYKLWVNDEL
IVQSDGVPYSGDVVNALTTSGKTAALSAGKTYTVKLEYQRVQGNFIPALGGLTGVQMSWA
SLRPPKDLSKYDAVVVATGTTSENEGEGSDHGFDLPDQQAELISFVAKANPNTIVVMHGG
GVANMQPWANKVGATLQAWFPGQQGGQALAEILYGKVNPSGKLPVTIDKNIEDNPSYASY
PDPAAYRGSNPLTEMTYSEGLYMGYRGYDKKHAKPLYPFGYGLSYTTFSYSDLKLSTNVL
TPGSTIDVKFTVTNTGDKAGFEVAQLYVQPVKPAVDRPEKELKGFTKVYLQPGESKTVSV
PIDSRSLAYYVDKTASWDVDAGKFKILVGADSENLTLNRTLITLYPEKLTTRDSNPLPLP
LRKAVQVSAAQTY