Protein Info for Psyr_2997 in Pseudomonas syringae pv. syringae B728a

Annotation: Glycoside hydrolase, family 13, N-terminal:Alpha amylase, catalytic region

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 727 TIGR02100: glycogen debranching enzyme GlgX" amino acids 29 to 720 (692 residues), 1099.7 bits, see alignment E=0 PF02922: CBM_48" amino acids 33 to 121 (89 residues), 60.3 bits, see alignment E=1.9e-20 PF00128: Alpha-amylase" amino acids 217 to 553 (337 residues), 49.6 bits, see alignment E=4.5e-17

Best Hits

KEGG orthology group: K02438, glycogen operon protein GlgX [EC: 3.2.1.-] (inferred from 100% identity to psb:Psyr_2997)

Predicted SEED Role

"Glycogen debranching enzyme (EC 3.2.1.-)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 3.2.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.-

Use Curated BLAST to search for 3.2.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZS41 at UniProt or InterPro

Protein Sequence (727 amino acids)

>Psyr_2997 Glycoside hydrolase, family 13, N-terminal:Alpha amylase, catalytic region (Pseudomonas syringae pv. syringae B728a)
MNTKSTTPAEDNSAPLDNPGSTPSRIREGLPFPLGASWDGLGVNFALFSANATKVELCLF
DSTGEIELERIELPEYTDEIYHGYLPDAHPGQIYGYRVYGPYDPKNGHRFNHNKLLIDPY
AKQLVGELKWSEALFGYTIGHPDGDLSFDERDSAPFVPKSKVIDEAYTWGRDQRVGTPWD
KTIFYETHVRGITMRHPEVAEELRGTFAGLGSAPVVEHIRKLGVTSVELLPIHAFVNDQH
LLQKGMTNYWGYNSIAFFAPDPRYLASGKIAEFKEMVAHLHHAGLEVILDVVYNHTAEGN
ELGPTLSMRGIDNASYYRLMPDDKRYYINDSGTGNTLDLSHPCVLQMVTDSLRYWASEMH
VDGFRFDLATILGRYHDGFDERHSFLVACRQDPVLRQVKLVAEPWDCGPGGYQVGGFPPG
WMEWNDKFRDTVRAFWKGDEAQLADFAARMTASGNMFNQRGRRPQASVNFITAHDGFTLH
DLVSYNDKHNEANDENNQDGSNNNLSWNHGVEGPTEDPEINSLRLRQMRNFFATLLLAQG
TPMIVAGDEFARTQHGNNNAYCQDSEIGWINWDLDEDGEALLKFVTRVIKLRQTYPILRR
SRFLVGDYNEEIGVKDVTWLAPDGNEMSVEQWHDANGRCLGMLMDGRAQETGIRRAGADA
TLLLVVNAHHDGVNFTLPEVPEGTHYECLIDTNQDDARSKEHFAFGSQYTVTPRSLLLFE
LKREDQQ