Protein Info for Psyr_2994 in Pseudomonas syringae pv. syringae B728a

Annotation: Glycoside hydrolase, family 77

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 692 TIGR00217: 4-alpha-glucanotransferase" amino acids 115 to 670 (556 residues), 316.7 bits, see alignment E=1.8e-98 PF02446: Glyco_hydro_77" amino acids 158 to 661 (504 residues), 372.1 bits, see alignment E=2.3e-115

Best Hits

KEGG orthology group: K00705, 4-alpha-glucanotransferase [EC: 2.4.1.25] (inferred from 100% identity to psb:Psyr_2994)

Predicted SEED Role

"4-alpha-glucanotransferase (amylomaltase) (EC 2.4.1.25)" in subsystem Glycogen metabolism or Maltose and Maltodextrin Utilization (EC 2.4.1.25)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZS44 at UniProt or InterPro

Protein Sequence (692 amino acids)

>Psyr_2994 Glycoside hydrolase, family 77 (Pseudomonas syringae pv. syringae B728a)
MNNEQLERLATEAGLSVHWVDANARPQTVSPDVLRKVLEALGYPAENGEAIDASLLRLQK
ASHGASAPPLLTVDVESNLDLSQWFAPQTPFTLHLEDGSSLDARLTSNAELPALAPPGYQ
QLEIAGQHLTIAVAPKTCFSMAMATDTSKPHGWGLTAQLYSLRREGDGGFGDTEALEKVL
RSAGERGADALGISPIHAMFANDPHRYSPYSPSSRLFLNSLYASPGAILGESAWRQAIED
ARVGDEMKRLETQTLIDWPAAANAKWKALHALYEGFSAGDHPLHEDFNSFRHSGGEALEN
HCRFEALCAESETLNIGNNWHEWPEQFKDPRSDAVAAFAKSHREQISFHAFAQWLIARGL
ERAQVAARSSGMRVGLIADLAVGADGAGSQAWSRQDELLSALTVGAPPDILNRAGQSWGI
SAFSPDGLKRNGFRAFIEMLRANFAHAGGLRIDHVMGLQRLWVIPQGAPPSEGAYLNFPL
DDMLRLLSLESWRHKAIVLGEDLGTVPEGLSEKLSARAILGMRVLLFEQNNGQFRPILDW
SDQALATTSTHDLPTLAGWLSELDIEWNARLGHIDEQHESQWREERTREYESLRRALSQN
IDSLPSETEDPAQIIDASISYLGHTRAPLVLLPIEDALGVEEQANLPGTIDSHPNWRRRL
PGDAASLLDNPNAARRLELLSRSRLQATERDQ