Protein Info for Psyr_2716 in Pseudomonas syringae pv. syringae B728a

Annotation: Mandelate racemase/muconate lactonizing enzyme:Mandelate racemase/muconate lactonizing enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 424 PF02746: MR_MLE_N" amino acids 32 to 135 (104 residues), 38.8 bits, see alignment E=9.6e-14 PF13378: MR_MLE_C" amino acids 174 to 387 (214 residues), 196.6 bits, see alignment E=4.6e-62

Best Hits

KEGG orthology group: K01706, glucarate dehydratase [EC: 4.2.1.40] (inferred from 100% identity to psb:Psyr_2716)

Predicted SEED Role

"mandelate racemase family protein Pfl_3283"

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.40

Use Curated BLAST to search for 4.2.1.40

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZSW7 at UniProt or InterPro

Protein Sequence (424 amino acids)

>Psyr_2716 Mandelate racemase/muconate lactonizing enzyme:Mandelate racemase/muconate lactonizing enzyme (Pseudomonas syringae pv. syringae B728a)
MKIIRVTVTPIAFRDPPLLNASGIHEPFALRSIIEVESDNGYIGLGESYGDAPALAIQQQ
VQTQLIGLDPFNLNQLRSIVQATVAAHKPASLAGAELAPGSHASKAVSNAYSAFEVAFLD
LQARSMNLPLVDLLGGAIRDQIPFSAYLFFKYAQHADAPYAPDSWGEALSEEQIVAQARR
MIDAYGFKSIKLKAGALQPEHEVSCIKALKKAFPGYPLRIDPNGNWSLETSIRMAELLGD
DLQYYEDPTPGLDGMSELHKRTGLPLATNMVVTDFDEFRRSVALDSVQIVLADHHYWGGL
RDTQALAKMCETFGLGVSMHSNSHLGISLMAMAHVAASVPNLDYACDTHYPWQEPDEEVI
KGGKLPIVDGCVSITRAPGMGLELDYDQLGKLNDQYHSCGIRQRDDVKQMQKYNPDWKAV
KPRY