Protein Info for Psyr_2691 in Pseudomonas syringae pv. syringae B728a

Annotation: Formyl transferase, N-terminal:Formyl transferase, C-terminal

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 664 PF00551: Formyl_trans_N" amino acids 27 to 178 (152 residues), 94.1 bits, see alignment E=1.8e-30 PF02911: Formyl_trans_C" amino acids 205 to 285 (81 residues), 70.6 bits, see alignment E=2.1e-23 PF01370: Epimerase" amino acids 322 to 569 (248 residues), 146.4 bits, see alignment E=2e-46 PF16363: GDP_Man_Dehyd" amino acids 323 to 647 (325 residues), 80.8 bits, see alignment E=2.8e-26

Best Hits

Swiss-Prot: 100% identical to ARNA_PSEU2: Bifunctional polymyxin resistance protein ArnA (arnA) from Pseudomonas syringae pv. syringae (strain B728a)

KEGG orthology group: K10011, UDP-4-amino-4-deoxy-L-arabinose formyltransferase / UDP-glucuronic acid dehydrogenase (UDP-4-keto-hexauronic acid decarboxylating) [EC: 1.1.1.305 2.1.2.13] (inferred from 100% identity to psb:Psyr_2691)

MetaCyc: 69% identical to fused UDP-4-amino-4-deoxy-L-arabinose formyltransferase/UDP-glucuronate dehydrogenase (Escherichia coli K-12 substr. MG1655)
RXN0-1862 [EC: 2.1.2.13]; RXN0-1861 [EC: 2.1.2.13, 1.1.1.305]

Predicted SEED Role

"UDP-4-amino-4-deoxy-L-arabinose formyltransferase (EC 2.1.2.13) / UDP-glucuronic acid oxidase (UDP-4-keto-hexauronic acid decarboxylating) (EC 1.1.1.305)" (EC 1.1.1.305, EC 2.1.2.13)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.305 or 2.1.2.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZSZ2 at UniProt or InterPro

Protein Sequence (664 amino acids)

>Psyr_2691 Formyl transferase, N-terminal:Formyl transferase, C-terminal (Pseudomonas syringae pv. syringae B728a)
MSTKAVVFAYHDIGCVGLQALLDAGYEIAAVFTHADDPREKTFFGSVAQLCARHGIAVHA
PEDPNHPLWVERIGKLAPDFIFSFYYRQLLGDSLLACAKKAALNLHGSLLPRYRGRAPAN
WVLVNGESETGVTLHQMVKRADAGPIVAQQRVSISATDTALTLHGKLRDAAADLLCETLP
LLAAQGQLPATPQDESRATYFGRRTPADGLIDWSLPATQLYNLIRAVTQPYPGAFCPVGD
NKLIVWAASVDTSSNGEAPGTVISHEPLRIACGEGSLVITAGQRGDNGLYLSGEQLAREF
GLVAGSQMLDKAKRRSVRRTRVLILGVNGFIGNHLSERLLQDDRYEIYGMDIGSDAIERL
RAKPNFHFIEGDISIHTEWIEYHIKKCDVVLPLVAIATPIEYTRNPLRVFELDFEENLKI
VRYCVKYNKRVIFPSTSEVYGMCQDANFNEDTSNLIVGPINKQRWIYSVSKQLLDRVIWA
YGQKGLQFTLFRPFNWMGPRLDRLDSARIGSSRAITQLILHLVEGTPIRLVDGGAQKRCF
TDVVDGIEALARIIENRDGRCNGQIINIGNPDNEASIRQLGEELLRQFEAHPLRGHFPPF
AGFREVESQSFYGKGYQDVSHRTPSIDNAKKLIGWTPGIELSETIGKTLDFFLREAMAEK
ADQC