Protein Info for Psyr_2566 in Pseudomonas syringae pv. syringae B728a

Annotation: dihydroxyacetone kinase DhaK subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02733: Dak1
  • amino acids 17 to 326 (310 residues), 386.7 bits, E=3.4e-120

Best Hits

Swiss-Prot: 43% identical to DHAK2_LISIN: PTS-dependent dihydroxyacetone kinase 2, dihydroxyacetone-binding subunit DhaK (dhaK-2) from Listeria innocua serovar 6a (strain ATCC BAA-680 / CLIP 11262)

KEGG orthology group: K05878, dihydroxyacetone kinase, N-terminal domain [EC: 2.7.1.-] (inferred from 100% identity to psb:Psyr_2566)

Predicted SEED Role

"Putative dihydroxyacetone kinase (EC 2.7.1.29), dihydroxyacetone binding subunit" in subsystem Dihydroxyacetone kinases (EC 2.7.1.29)

MetaCyc Pathways

• formaldehyde assimilation III (dihydroxyacetone cycle) (11/12 steps found)
• glycerol degradation II (1/2 steps found)
• glycerol degradation to butanol (11/16 steps found)
• superpathway of glycerol degradation to 1,3-propanediol (1/4 steps found)

KEGG Metabolic Maps

• Glycerolipid metabolism
• Lipopolysaccharide biosynthesis
• Nicotinate and nicotinamide metabolism
• Pentose phosphate pathway

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.-, 2.7.1.29

Use Curated BLAST to search for 2.7.1.- or 2.7.1.29

Sequence Analysis Tools

See Q4ZTB7 at UniProt or InterPro

Protein Sequence (333 amino acids)

>Psyr_2566 dihydroxyacetone kinase DhaK subunit (Pseudomonas syringae pv. syringae B728a)
MNRVINDPDQVVEDMLRGILVAHPELSQSEGNPRVISKTRPSDQGRVGIVTGGGSGHEPA
FLGYVGPGLVDAVAVGEIFSSPTAKSFFDAFRAADHGAGIACLYGNYAGDNMNVKLAMKM
AASKDLQVRTVVANDDVASAPKSDIAKRRGVAGEIFMWKIGGAAAANGYDLDGVIRVAQK
AVDQCRSIGIGLTPCTIAAVGKPNFQIPDGKMELGIGHHGEPGIEVIDIEPAAAMAERML
EPILADRDFSVDDSVVVLVSGLGATPVMELYVFYAEVERLLKARGLKIHRCYVGNYFTSL
EMMGVTLTLLGLDAELKTLIDHPCRSIGMTQAE