Protein Info for Psyr_2557 in Pseudomonas syringae pv. syringae B728a

Annotation: Phosphoenolpyruvate synthase/pyruvate phosphate dikinase-like protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01326: PPDK_N
  • amino acids 80 to 203 (124 residues), 92.9 bits, E=2.4e-30
  • amino acids 230 to 274 (45 residues), 32.1 bits, 7.9e-12
• PF00391: PEP-utilizers
  • amino acids 551 to 596 (46 residues), 26.7 bits, 3.6e-10

Best Hits

KEGG orthology group: None (inferred from 100% identity to psb:Psyr_2557)

Predicted SEED Role

"Phosphoenolpyruvate synthase (EC 2.7.9.2)" in subsystem Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 2.7.9.2)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (23/26 steps found)
• gluconeogenesis I (13/13 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (14/17 steps found)
• glycolysis II (from fructose 6-phosphate) (9/11 steps found)
• glycolysis I (from glucose 6-phosphate) (10/13 steps found)
• superpathway of anaerobic sucrose degradation (14/19 steps found)
• glycolysis V (Pyrococcus) (7/10 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (13/19 steps found)
• superpathway of hexitol degradation (bacteria) (12/18 steps found)
• superpathway of N-acetylneuraminate degradation (12/22 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (9/18 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 2.7.9.2

Use Curated BLAST to search for 2.7.9.2

Sequence Analysis Tools

See Q4ZTC6 at UniProt or InterPro

Protein Sequence (631 amino acids)

>Psyr_2557 Phosphoenolpyruvate synthase/pyruvate phosphate dikinase-like protein (Pseudomonas syringae pv. syringae B728a)
MSSKYLTLKHLAGDLPVPALSALHDADYRVLWQDFANRKQAIEDTLKTVRITAGAFLDEH
MDRIARFAEFEWSDAADGHLQALLGQAGFDADSELAVRSSANIEDGNQHSFAGIFSTHLS
VKGLPALKAAILDVWRSGVSRQAILERLRASMLETPVEMTVIMQRMVKARFAGVAFSHDP
LTGEAVTLIETVASLGEAMVSGVERGMSARIGDQQLLDCDPALEPHTALLLEVAALASRA
SAALGMPADIEWAADEGQVWLLQARPVTSLREARSLEPVLEWAELYLASDEALTEFRPLP
AFAQYFRSKRRPLALFAQTHDVSAGQALLIKANRQGLLEPTMSQALLDRLKTDQVVLDFS
DQVRQQILERGALRERLLELSDERVVCFVLREFLQGETGLITQACDDGSTTCEWSSEGLL
AINRGIAGTQRARLATNDPSGPADAPQLHSITLAAGALLGSVQLEWVRAGQQLHLIDFSP
LSTFVQVQDATEIRLISPGYADGPVVLVTAGRQLEELSISATVSINSIPSSSSLGAELEE
LERQLRVHDGQAIVVSPRPYAALAALLPFARGFVFEQASMLCHLSILLREHGIPAVESEA
LYQQGLAGQRITFSHSPKALPHAVNEVRELQ