Protein Info for Psyr_2489 in Pseudomonas syringae pv. syringae B728a

Annotation: Alpha amylase, catalytic region

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 672 PF11896: GlgE_dom_N_S" amino acids 31 to 213 (183 residues), 179.6 bits, see alignment E=1.1e-56 PF00128: Alpha-amylase" amino acids 237 to 342 (106 residues), 26.9 bits, see alignment E=5.5e-10 PF21702: GLGE_C" amino acids 585 to 661 (77 residues), 62.6 bits, see alignment E=5e-21

Best Hits

Swiss-Prot: 55% identical to GLGE_XANCP: Alpha-1,4-glucan:maltose-1-phosphate maltosyltransferase (glgE) from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)

KEGG orthology group: None (inferred from 100% identity to psb:Psyr_2489)

Predicted SEED Role

"Alpha-amylase (EC 3.2.1.1)" in subsystem Maltose and Maltodextrin Utilization or Trehalose Biosynthesis (EC 3.2.1.1)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZTJ4 at UniProt or InterPro

Protein Sequence (672 amino acids)

>Psyr_2489 Alpha amylase, catalytic region (Pseudomonas syringae pv. syringae B728a)
MNEQPYGPDSLATDVPQATLPLSLTQALQLPRIVIEDTLPVIDGGLFAAKAIIGQPVTVT
SKVYADGHDKMAVNIRWRAADEEHWHSAPMRELGNDSWVGEFTPTSVSRHVFRLEAWIDQ
FGSYRYELEKKFGAGVPVDLELEEGRIHLVHAAERSQNEQRQQIEGLLGQLEQGDKDEKV
ALLLHSETASLMKLADNQAFLSRSVEFPLDVERELAQFASWYELFPRSMTDDPARHGTFN
DVHSRLPIIRDMGFDVLYFPPIHPIGRAHRKGPNNSLTAGPDDPGSPYAIGSEDGGHEAI
HPQLGSREDFRHLVKAAAEHGLEIALDFAIQCSQDHPWLKQHPGWFSWRPDGTIRYAENP
PKKYQDIVNVDFYAPDAIPGLWTELRDIVLGWVNEGVKIFRVDNPHTKPLPFWQWMIGEV
RSQHPEVMFLAEAFTKPAMMARLGKVGYSQSYTYFTWRNTKAELSEYFTQLNEVPWRDCY
RPNFFVNTPDINPRFLHESGRPGFLIRAALATMGSGLWGMYSGFELCEAAPIPGKEEYLD
SEKYQIRVRDYAAPGNIIAEIAQLNRIRRQNPALQTHLGLKLYNAWNDNILYFGKRSADG
SNFILVAVNLDPYNAQEADFELPLWEMGLPDDAATSGEDLMTGHRWTWYGKYQHTRLDPS
QPFGIWRIQAAQ