Protein Info for Psyr_2474 in Pseudomonas syringae pv. syringae B728a

Annotation: Acyl-CoA dehydrogenase, C-terminal:Acyl-CoA dehydrogenase, central region

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 409 PF02771: Acyl-CoA_dh_N" amino acids 8 to 128 (121 residues), 42.7 bits, see alignment E=1.4e-14 PF02770: Acyl-CoA_dh_M" amino acids 132 to 233 (102 residues), 73.8 bits, see alignment E=2e-24 PF00441: Acyl-CoA_dh_1" amino acids 245 to 393 (149 residues), 110.5 bits, see alignment E=1.8e-35 PF08028: Acyl-CoA_dh_2" amino acids 262 to 367 (106 residues), 38.4 bits, see alignment E=2.9e-13

Best Hits

KEGG orthology group: K00249, acyl-CoA dehydrogenase [EC: 1.3.99.3] (inferred from 100% identity to psb:Psyr_2474)

Predicted SEED Role

"Isovaleryl-CoA dehydrogenase (EC 1.3.8.4)" (EC 1.3.8.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.8.4, 1.3.99.3

Use Curated BLAST to search for 1.3.8.4 or 1.3.99.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZTK9 at UniProt or InterPro

Protein Sequence (409 amino acids)

>Psyr_2474 Acyl-CoA dehydrogenase, C-terminal:Acyl-CoA dehydrogenase, central region (Pseudomonas syringae pv. syringae B728a)
MDFAYSPKVQALRERVTAFMDAYVYPAEPVFERQVAEGDRWQPTAIMEELKARARSEGLW
NLFLPDSGLGAGLSNLEYAPLAEIMGRSMLGPEPFNCSAPDTGNMEVLVRYASEEQKARW
LEPLLRGEIRSAFAMTEPDVASSDATNMAARAERDGDQWVINGRKWWTSGACDPRCKILI
FMGLSNPEGPRHQQHSMILVPVDTPGVKIVRPLPVFGYDDAPHGHAEVLFENVRVPFENV
LLGEGRGFEIAQGRLGPGRIHHCMRSIGMAERALELMCRRSIDRVAFGKPLARLGGNIDL
IADSRMEIDMARLLTLKAAYMMDTVGNKVARSEIAQIKVVAPNVALKVIDRAIQMHGAAG
ISSDFPLAYMYAMQRTLRLADGPDEVHRAAVGKYELGKYMPADSTRSRS