Protein Info for Psyr_2462 in Pseudomonas syringae pv. syringae B728a

Annotation: Nitrite/sulfite reductase, hemoprotein beta-component, ferrodoxin-like:Nitrite and sulphite reductase 4Fe-4S region

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03460: NIR_SIR_ferr
  • amino acids 53 to 109 (57 residues), 61.8 bits, 4.2e-21
  • amino acids 346 to 398 (53 residues), 43.1 bits, 2.9e-15
• PF01077: NIR_SIR
  • amino acids 119 to 272 (154 residues), 166.9 bits, E=2.5e-53
  • amino acids 412 to 547 (136 residues), 55.9 bits, E=3.5e-19

Best Hits

KEGG orthology group: K00381, sulfite reductase (NADPH) hemoprotein beta-component [EC: 1.8.1.2] (inferred from 100% identity to psb:Psyr_2462)

Predicted SEED Role

"Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2)" in subsystem Cysteine Biosynthesis (EC 1.8.1.2)

MetaCyc Pathways

• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (7/10 steps found)

KEGG Metabolic Maps

• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.2

Use Curated BLAST to search for 1.8.1.2

Sequence Analysis Tools

See Q4ZTM1 at UniProt or InterPro

Protein Sequence (552 amino acids)

>Psyr_2462 Nitrite/sulfite reductase, hemoprotein beta-component, ferrodoxin-like:Nitrite and sulphite reductase 4Fe-4S region (Pseudomonas syringae pv. syringae B728a)
MYVYDDYDQKIIEDRVKQFRDQTRRYLVGELSEEEFRPLRLQNGLYVQRFAPMLRVAVPY
GQLTSRQARMMAKIARDYDKGYAHISTRQNVQFNWPALEDVPDILAELATVQMHAIQTSG
NCLRNVTTDQFAGVAADELVDPRPWCEIVRQWTTFHPEFAYLPRKFKIAINGSTSDRAAI
EVHDIGLEPLRNEAGELGFRVLVGGGLGRTPVVGAFINEFLPWQDLLSYLDAILRVYNRY
GRRDNKYKARIKILVKALTPEVFAAKVEAEMAHLRGGQTTLTEAEVQRVSRHFVDPQYKV
LSDQHAELAALDAQHPGFARWRQRNVLAHKKPGYIAVTLSLKPTGVAPGDVTDKQLDAIA
DLADRYSFGQLRTSHEQNIILADVEQRELFTLWNELRDNGFATPNIGLLTDIICCPGGDF
CSLANAKSIPIAESIQRRFDDLDYLFDIGELDLNISGCMNACGHHHVGHIGVLGVDKKGE
EFYQVSLGGSASRDASLGKILGPSFAQEAMPDVIEKLIDVYVEKRTEDERFIDTYQRIGI
DPFKERVYAANH