Protein Info for Psyr_2396 in Pseudomonas syringae pv. syringae B728a

Annotation: Tartrate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02089: tartrate dehydrogenase
  • amino acids 14 to 369 (356 residues), 553.7 bits, E=7.4e-171
• PF00180: Iso_dh
  • amino acids 17 to 364 (348 residues), 297.1 bits, E=1e-92

Best Hits

Swiss-Prot: 84% identical to TTUC_PSEPU: Tartrate dehydrogenase/decarboxylase from Pseudomonas putida

KEGG orthology group: K07246, tartrate dehydrogenase/decarboxylase / D-malate dehydrogenase [EC: 1.1.1.83 1.1.1.93 4.1.1.73] (inferred from 100% identity to psb:Psyr_2396)

MetaCyc: 84% identical to tartrate dehydrogenase subunit (Pseudomonas putida)
4.1.1.M27 [EC: 4.1.1.M27]; Tartrate dehydrogenase. [EC: 4.1.1.M27, 1.1.1.93, 4.1.1.73]; D-malate dehydrogenase (decarboxylating). [EC: 4.1.1.M27, 1.1.1.93, 4.1.1.73, 1.1.1.83]

Predicted SEED Role

"Tartrate dehydrogenase (EC 1.1.1.93) / Tartrate decarboxylase (EC 4.1.1.73) / D-malic enzyme (EC 1.1.1.83)" in subsystem Pyruvate metabolism I: anaplerotic reactions, PEP (EC 1.1.1.83, EC 1.1.1.93, EC 4.1.1.73)

MetaCyc Pathways

• D-malate degradation (1/1 steps found)
• gentisate degradation II (3/4 steps found)
• methylgallate degradation (2/6 steps found)
• protocatechuate degradation I (meta-cleavage pathway) (3/8 steps found)
• superpathway of vanillin and vanillate degradation (4/10 steps found)
• syringate degradation (3/12 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Glyoxylate and dicarboxylate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.83 or 1.1.1.93 or 4.1.1.73 or 4.1.1.M27

Sequence Analysis Tools

See Q4ZTT7 at UniProt or InterPro

Protein Sequence (373 amino acids)

>Psyr_2396 Tartrate dehydrogenase (Pseudomonas syringae pv. syringae B728a)
MDRFRESAAMNNNKLRIAAIAGDGIGLEVLPEGVRVVQAAAARHGLELEFEYFEWASCDY
YLKHGKMMPDDWFEQLKGFDSIFFGAVGWPEKVPDHISLWGSLLKFRREFDQYANIRPVR
LFPGVPCPLANRKPGDIDFVVVRENTEGEYSSLGGIMFENTENEFVLQESVFTRRGVDRI
LKFAFEMASKRERKHVTSATKSNGMAISMPYWDKRTEAMASQYPGISWDKQHIDILCARF
VLQPERFDVVVASNLFGDILSDLGPACAGTIGIAPSANLNPERHFPSLFEPVHGSAPDIF
GQNIANPIAMIWSGALMLEFLGQGDQRFTAAHDEIITAIEQVIANGDVTPDLGGTLSTQH
VGAAIAERVSAGH