Protein Info for Psyr_2361 in Pseudomonas syringae pv. syringae B728a

Annotation: DNA damage-inducible DNA polymerase II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 787 PF21474: DNApolII_N" amino acids 6 to 41 (36 residues), 73.6 bits, see alignment (E = 1.2e-24) PF03104: DNA_pol_B_exo1" amino acids 78 to 298 (221 residues), 41.9 bits, see alignment E=1.1e-14 PF00136: DNA_pol_B" amino acids 381 to 740 (360 residues), 129 bits, see alignment E=3.4e-41

Best Hits

Swiss-Prot: 68% identical to DPO2_ECOLI: DNA polymerase II (polB) from Escherichia coli (strain K12)

KEGG orthology group: K02336, DNA polymerase II [EC: 2.7.7.7] (inferred from 76% identity to cti:RALTA_A1553)

MetaCyc: 68% identical to DNA polymerase II (Escherichia coli K-12 substr. MG1655)
3.1.11.-; DNA-directed DNA polymerase. [EC: 2.7.7.7]

Predicted SEED Role

"DNA polymerase II (EC 2.7.7.7)" in subsystem DNA-replication or DNA Repair Base Excision (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZTX2 at UniProt or InterPro

Protein Sequence (787 amino acids)

>Psyr_2361 DNA damage-inducible DNA polymerase II (Pseudomonas syringae pv. syringae B728a)
MDVQQGFILSRHWQDTPAGTEVDFWLATDQGPRHIRLPCQPSVAFIPAEQGERARSLLRT
ERGVELRPLELCDFRHRPVLGVYCQQHRQLMNIEKLLRRGNVDVYEADIRPPERYLMERF
ITAPVQFGGTPDADGVLCDAQIRPSPDYRPNLRLVSLDIETTARGELYSIALEGCGQRQV
YMLGPANGGDETLDFQLDYCDTRAQLLERLNDWLALHDPDAIIGWNVIQFDLRVLHEHAQ
RLQVPLRLGRGGEAMGWREHGSRNNHFFAEVSGRLIIDGIEALRSATWSFPSFSLEYVAQ
TLLGEGKAIDTPYQRMDEINRMFAEDKPALARYNIKDCELVSRIFAKTQLLTFLLERATV
TGLPADRSGGSVAAFCHLYIPLMHRQGFVAPNLGERLPEASPGGFVMDSQPGLYESVLVL
DYKSLYPSIIRTFLIDPVGLIEGLRHPADSESVPGFRGARFSRTRHSLPAIVERVWQGRE
AAKREQNAPLSQALKIIMNAFYGVLGSSGCRFFDPRLASSITLRGHEIMLKTRDLIQAQG
YTVIYGDTDSTFVWLGRPHGQEEAASIGKALVQQVNQWWREHLHTEYGLHSALELQYETH
FRRFLMPTVRGAEEGSKKRYAGLVTRADGSEEMVYKGLETVRTDWSPLAQQFQQELYLRI
FKRQPYQDYVRDYVQRTLSGELDDLLIYRKRLRRKLDDYQRNVPPHVRAARLADEYNDQQ
GRPRQYQSGGWISYVMTTAGPEPLEIRHAPIDYDHYVTRQLQPLADAILPFVDDDFSTLI
GGQLGLF