Protein Info for Psyr_2330 in Pseudomonas syringae pv. syringae B728a
Annotation: FAD dependent oxidoreductase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00303, sarcosine oxidase, subunit beta [EC: 1.5.3.1] (inferred from 100% identity to psb:Psyr_2330)Predicted SEED Role
"Sarcosine oxidase beta subunit (EC 1.5.3.1)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (EC 1.5.3.1)
MetaCyc Pathways
- glycine betaine degradation I (6/8 steps found)
- creatinine degradation I (1/4 steps found)
- creatinine degradation II (1/5 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.5.3.1
Use Curated BLAST to search for 1.5.3.1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q4ZU02 at UniProt or InterPro
Protein Sequence (394 amino acids)
>Psyr_2330 FAD dependent oxidoreductase (Pseudomonas syringae pv. syringae B728a) MSTLIETDAIVIGGGIVGASAALTLALKGKRVALLERDFCGSHSSGVNYGGVRRQGRPLS QLPLSQRAHQIWGNLRELIGIDGEYQRSGHLKLARSEQDMNALRAYAEASQGFGLDLQLL DRDQLRARYPWAGDVAVGASLCADDGHANPRLVSPAFARAARQAGALVFEQAPVSDVSHD GNVFVVTTATGLTLRATWLLNCAGAWAAALAAQFNEPVPMYSGYPAMLVTEPLPMFMDVS TGVEGGGIYARQVARGNCVLGGGQGFALDPARARPGQAAVLDILRNAVELYPPLAGAQAI RTWSGTEGYLPDREPVLGPSLNRPGLLHGFGFAGAGFQIGPAAGEALAEWVCDGASRISL DAFSIGRFQQIVLQPPAIEPVTNVIPLHRGRSSL