Protein Info for Psyr_2282 in Pseudomonas syringae pv. syringae B728a

Annotation: Hydrophobe/amphiphile efflux-1 HAE1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1047 signal peptide" amino acids 1 to 33 (33 residues), see Phobius details transmembrane" amino acids 342 to 361 (20 residues), see Phobius details amino acids 368 to 388 (21 residues), see Phobius details amino acids 394 to 415 (22 residues), see Phobius details amino acids 443 to 468 (26 residues), see Phobius details amino acids 475 to 499 (25 residues), see Phobius details amino acids 541 to 559 (19 residues), see Phobius details amino acids 869 to 888 (20 residues), see Phobius details amino acids 895 to 915 (21 residues), see Phobius details amino acids 921 to 945 (25 residues), see Phobius details amino acids 969 to 991 (23 residues), see Phobius details amino acids 1002 to 1026 (25 residues), see Phobius details TIGR00915: RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family" amino acids 1 to 1040 (1040 residues), 1409.5 bits, see alignment E=0 PF00873: ACR_tran" amino acids 1 to 1024 (1024 residues), 1148.8 bits, see alignment E=0 PF03176: MMPL" amino acids 345 to 498 (154 residues), 36.4 bits, see alignment E=2.9e-13 amino acids 833 to 1021 (189 residues), 20.8 bits, see alignment E=1.7e-08

Best Hits

Swiss-Prot: 51% identical to MEXB_PSEAE: Multidrug resistance protein MexB (mexB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 67% identity to axy:AXYL_03146)

Predicted SEED Role

"RND efflux system, inner membrane transporter CmeB" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZU47 at UniProt or InterPro

Protein Sequence (1047 amino acids)

>Psyr_2282 Hydrophobe/amphiphile efflux-1 HAE1 (Pseudomonas syringae pv. syringae B728a)
MSLFFIKRPNFAWVLALFILLAGLMALPSLPVAQYPDVAPPQITITATYPGASAKVLVDS
VTSVIEDELNGAKGMLYYESTSNSTGSAEINVTFVPGTNPDLAQVEVQNRIKKAEARLPQ
TVLSQGLQVEQASSGFLLIYTLNYKDGAASKDTVALADYAARNVNNEISRVNGVGRLQFF
AAEAAMRVWIDPQKLVGFGLSIDDVNAAIRAQNVQVPAGSFGSSPASSLQELTATLAVKG
TLDNPEEFGRIVLRANEDGSAVHLSDVARVAVGSQDYSFESRLNGQRAVAGAVQLSPGAN
AIQTARAVEQRLTELSVNFPEGVGFSIPYDTSRFVDVAIDKVIYTLIEAMVLVFLVMFLF
LQNIRYTLIPTIVVPVCLAGTLAIMYLMGFSVNMMTMFGMVLAIGILVDDAIVVVENVER
IMAEEGLSPAAATVKAMQQVSGAIFGITLVLAAVFLPLAFMGGSVGVIYQQFSLSLAVSI
LFSGFLALTFTPALCATLLKPIPAGHHEKRGFFGGFNRLFGKFTHRYERVSSSMIKRAGR
YMLLYVGIVGLLGFFYLRLPESFVPVEDQGYLIIDVQLPPGATRSRTDLTAQLLENYMLS
REATGAVTMLLGFSFSGMGENAGLAFPTLKDWSERAKGQSAAEEAVAFNQHFAGLGDGTV
MAVTPPPIDGLGTSGGFSLRLQDRAGLGREALLAARDKLLGEANGNPKILYAMMEGLAEA
PQLRLSIDREKARALGVSFESINNALSTAFGSSVISDFANAGRQQRVVVQAEQSARMTPE
SVLKLYVPNSSGTLVPLGAFVSTHWEQGPVQIARYNGYPAFRISGDAAPGVSTGEAMAEI
ERIVSKLPQGIGYEWTGLSYQERVASGQAAGLFGLALLVVFLLLVALYESWAIPLVVMLI
VPVGALGSVLAVTAVGMPNDVYFKVGLITIIGLAAKNAILIVEFAKELWDQGHSLRDAAL
QAARLRFRPIVMTSLAFILGVVPLTLATGAGAASQRAIGTGVIGGMLSATLLGVVLVPIF
FVWVLSVLRRKPHAQQANEDQQLPEKR