Protein Info for Psyr_2249 in Pseudomonas syringae pv. syringae B728a

Annotation: Binding-protein-dependent transport systems inner membrane component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 262 transmembrane" amino acids 19 to 36 (18 residues), see Phobius details amino acids 76 to 102 (27 residues), see Phobius details amino acids 123 to 146 (24 residues), see Phobius details amino acids 182 to 200 (19 residues), see Phobius details amino acids 207 to 225 (19 residues), see Phobius details amino acids 231 to 252 (22 residues), see Phobius details TIGR01097: phosphonate ABC transporter, permease protein PhnE" amino acids 18 to 261 (244 residues), 310.1 bits, see alignment E=5.8e-97 PF00528: BPD_transp_1" amino acids 91 to 256 (166 residues), 73 bits, see alignment E=1.3e-24

Best Hits

Swiss-Prot: 70% identical to PHNE_ECOBD: Phosphonate transport system permease protein PhnE (phnE) from Escherichia coli (strain B / BL21-DE3)

KEGG orthology group: K02042, phosphonate transport system permease protein (inferred from 100% identity to psb:Psyr_2249)

Predicted SEED Role

"Phosphonate ABC transporter permease protein phnE (TC 3.A.1.9.1)" in subsystem ABC transporter alkylphosphonate (TC 3.A.1.9.1) (TC 3.A.1.9.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZU80 at UniProt or InterPro

Protein Sequence (262 amino acids)

>Psyr_2249 Binding-protein-dependent transport systems inner membrane component (Pseudomonas syringae pv. syringae B728a)
MTTHVAYTQVAGKPNRSRYLGWGLLLAALAWAWHGAEMNPMALVRDSSNMATFAADFFPP
DFREWRSYLKEMLVTIQIALWGTALAIVCSIPLGILCAENITPWWIHLPLRRCMDAFRSI
NEMVFAMLFVVAVGLGPFAGVLALWISTTGVLAKLFAEAVEAIEPGPVEGVRATGASALQ
EVIYGVIPQVMPLWISYALYRFESNVRSATVVGMVGAGGIGVILWENIRAFAFVQTSAVL
LVIIVVVSVIDIMSQRLRKQFI