Protein Info for Psyr_2179 in Pseudomonas syringae pv. syringae B728a

Annotation: General substrate transporter:Major facilitator superfamily

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 458 transmembrane" amino acids 27 to 52 (26 residues), see Phobius details amino acids 65 to 89 (25 residues), see Phobius details amino acids 97 to 116 (20 residues), see Phobius details amino acids 122 to 145 (24 residues), see Phobius details amino acids 166 to 186 (21 residues), see Phobius details amino acids 198 to 217 (20 residues), see Phobius details amino acids 247 to 265 (19 residues), see Phobius details amino acids 284 to 306 (23 residues), see Phobius details amino acids 313 to 333 (21 residues), see Phobius details amino acids 340 to 362 (23 residues), see Phobius details amino acids 374 to 395 (22 residues), see Phobius details amino acids 408 to 427 (20 residues), see Phobius details PF00083: Sugar_tr" amino acids 30 to 224 (195 residues), 93.8 bits, see alignment E=1.1e-30 amino acids 225 to 422 (198 residues), 26.7 bits, see alignment E=2.7e-10 PF07690: MFS_1" amino acids 43 to 280 (238 residues), 54.1 bits, see alignment E=1.3e-18 amino acids 277 to 426 (150 residues), 36.8 bits, see alignment E=2.2e-13

Best Hits

Swiss-Prot: 58% identical to KGTP_ECOLI: Alpha-ketoglutarate permease (kgtP) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to psb:Psyr_2179)

MetaCyc: 58% identical to alpha-ketoglutarate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-23

Predicted SEED Role

"Alpha-ketoglutarate permease"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZUF0 at UniProt or InterPro

Protein Sequence (458 amino acids)

>Psyr_2179 General substrate transporter:Major facilitator superfamily (Pseudomonas syringae pv. syringae B728a)
MSASTSDSTRHTEKSDQMKSPNRVRAIMGACSGNLVEWYDFFIYAYTAIYFASSFFPKGD
TTSQLLATAGVFAVGFFMRPLGGWIFGWIADTRGRKVSMIISVFMMCAGSLLIAVMPTYE
TIGVAAPVLLVVARLIQGLSVGAEYGTGATYISEIATPGRRCFYGSFQYFTIIAGQLLAL
MTVVILQQTLTGEELREWGWRIPFFIGAFSSIVVVYLRRAMHETATKKEMNRKDAGSLRG
MFKHKRAVALVVAFTIGGSLYFYTFTTYMQKFLVISAGFSPETVSFIMTAALVGFMFCQP
LFGLLADRIGIKVHMLLFSGLAMLLVIPLLYSLQSVSSPFTAFLLVFGGLAIASLYTPIA
GIVKAELFPPAVRALGVGFPYAVGNAAFGGTAEYVALSLRSQGIEEYFFFYVAAVVTITF
IAATLMPNLSRYGYISGSGEIEERTGLRGCIKSQAARI