Protein Info for Psyr_2171 in Pseudomonas syringae pv. syringae B728a

Annotation: HpcH/HpaI aldolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03328: HpcH_HpaI
  • amino acids 7 to 214 (208 residues), 98.5 bits, E=3.5e-32
• PF15617: C-C_Bond_Lyase
  • amino acids 191 to 270 (80 residues), 34.4 bits, E=1.4e-12

Best Hits

KEGG orthology group: K01644, citrate lyase subunit beta / citryl-CoA lyase [EC: 4.1.3.34 4.1.3.6] (inferred from 100% identity to psb:Psyr_2171)

Predicted SEED Role

"Citrate lyase beta chain (EC 4.1.3.6)" (EC 4.1.3.6)

MetaCyc Pathways

• citrate degradation (2/2 steps found)
• reductive TCA cycle II (6/12 steps found)

KEGG Metabolic Maps

• Citrate cycle (TCA cycle)

Isozymes

Compare fitness of predicted isozymes for: 4.1.3.6

Use Curated BLAST to search for 4.1.3.34 or 4.1.3.6

Sequence Analysis Tools

See Q4ZUF8 at UniProt or InterPro

Protein Sequence (271 amino acids)

>Psyr_2171 HpcH/HpaI aldolase (Pseudomonas syringae pv. syringae B728a)
MSKSLVRSALFVPATRPERISKAIATGADRVIVDLEDAVQENLKEQARDNLDAFLSEAPD
ARILVRVNAPDHWAHVADLDLCRRHAGVIGILLPKAESAKQVTTAAGTEKPIWPIIESAK
GLSALNEIAGTQGVERLSFGSLDLGLDLNLTTGSPAAEEILSHARYAVLLATRVAALAPA
LDGVFPSFQDTAGLLRAVHFARDMGFGGALCIHPTQVAIIHQALKPSQEELQWAQRIIEA
ARSGEGVFVLDGQMVDAPVIGRAQSILARAI