Protein Info for Psyr_2162 in Pseudomonas syringae pv. syringae B728a

Annotation: Ser-tRNA(Thr) hydrolase / threonyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 640 PF02824: TGS" amino acids 3 to 61 (59 residues), 46.6 bits, see alignment 5.9e-16 TIGR00418: threonine--tRNA ligase" amino acids 72 to 629 (558 residues), 743.4 bits, see alignment E=1.1e-227 PF07973: tRNA_SAD" amino acids 171 to 218 (48 residues), 54.6 bits, see alignment 1.7e-18 PF00587: tRNA-synt_2b" amino acids 317 to 528 (212 residues), 178 bits, see alignment E=3.9e-56 PF03129: HGTP_anticodon" amino acids 540 to 628 (89 residues), 73.8 bits, see alignment E=2.1e-24

Best Hits

Swiss-Prot: 100% identical to SYT_PSEU2: Threonine--tRNA ligase (thrS) from Pseudomonas syringae pv. syringae (strain B728a)

KEGG orthology group: K01868, threonyl-tRNA synthetase [EC: 6.1.1.3] (inferred from 100% identity to psb:Psyr_2162)

MetaCyc: 66% identical to threonine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Threonine--tRNA ligase. [EC: 6.1.1.3]; 6.1.1.3 [EC: 6.1.1.3]; 3.1.1.- [EC: 6.1.1.3]

Predicted SEED Role

"Threonyl-tRNA synthetase (EC 6.1.1.3)" (EC 6.1.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZUG7 at UniProt or InterPro

Protein Sequence (640 amino acids)

>Psyr_2162 Ser-tRNA(Thr) hydrolase / threonyl-tRNA synthetase (Pseudomonas syringae pv. syringae B728a)
MPTITLPDGSQRSFDQAVSVADVALSIGAGLAKATVAGKVDGKLVDACDLIENDASLQII
TPKDQEGLEIIRHSCAHLVGHAVKQLYPTAKMVIGPVIDDGFYYDIAYERPFTPDDMAAI
EQRMQQLIEKDYDVIKKVTPRAEVIEVFTARHEDYKLRLVEDMPSEQAMGLYYHEEYVDM
CRGPHVPNTRFLKSFKLTKLSGAYWRGDAKNEQLQRVYGTAWADKKQLAAYIQRIEEAEK
RDHRKIGKRLGLFHTQEEAPGMVFWHPQGWTLYQVLEQYMRKVQRENGYLEIKTPQVVDR
SLWEKSGHWANYADNMFTTESESRDYAIKPMNCPCHVQVFNQGLKSYRELPMRLAEFGAC
HRNEPSGALHGIMRVRGFTQDDAHIFCTEDQMQAESAAFIKLTLDVYADFGFKDIELKLS
TRPEKRVGSDELWDRAESALASALDSAGLPYDLQPGEGAFYGPKIEFSLKDCLGRVWQCG
TLQLDFNLPIRLSAEYVSEDNSRKNPVMLHRAILGSFERFIGILIEHYEGAFPAWLAPTQ
AVIMNITDKQADFALEVEKTLAESGFRAKSDLRNEKIGFKIREHTLLKVPYLLVIGDREV
EMQTVAVRTREGADLGSMPVAQFAEFLAQAVSRRGRQDTE